Protein expression can be used to measure the way in which proteins are synthesized, modified and regulated in living cells. The modulation of protein expression by project compounds can provide powerful information to help guide your discovery program. At Sygnature Discovery we have experience in measuring protein expression levels using a variety of assay formats.
Established techniques at Sygnature for the measurement of protein expression levels include:
- Dot Blot
- Western Blot
- Traditional ELISAs
Dot-blot techniques can be used to visualise and measure protein expression levels from large volume lysate loads, this has particular benefit if the target of interest is only present at low levels within the sample.
- 96-well manifold allows for higher sample throughput than a traditional SDS-PAGE based analysis.
- Advantageous when analysing compounds IC50 values or optimising conditions.
- Enables direct loading from the culture plate.
Western blotting is a routine technique for protein analysis, where the specificity of the labelled antibody-epitope interaction enables a target protein to be identified within the midst of a complex protein mixture.
Western blotting can produce qualitative and semi-quantitative data about the protein of interest, detected using our ChemiDoc™ MP Gel Imaging System.