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High Content Imaging and 3D Cultures

Our state-of-the-art ImageXpress® Micro Confocal (IXM-C) High-Content Imaging platform provides multi-dimensional, High-throughput Screening solutions to facilitate compound profiling, MOA analysis and; through the use of highly relevant in vitro models, to support interrogation of compound safety.

The IXM-C allows our scientists to capture high-quality images with a wide range of objective lenses, and the possibility to multiplex up to 5 wavelengths together with transmitted light. The imaging mode options of widefield and confocal provide the sensitivity and resolution required for a given assay. Combined with MetaXpress® High-Content Power Core Image Acquisition and Analysis Software, the ImageXpress® Micro Confocal system provides a complete multi-dimensional, high-throughput screening solution.

3D cultures more closely resemble the in vivo tissue environment due to the promotion of cell-cell contacts, re-establishment of cell polarity and the production of extracellular matrices. At Sygnature Discovery we offer expertise in the high content multi-parametric imaging/screening of 3D cell cultures using the IXM-C platform for the evaluation of compound dependent effects on a number of clinically relevant parameters.

A range of assay formats are available and are applicable to multiple phases of the drug discovery process, including target identification, hit validation and candidate drug profiling.

Current assays available include:

  • Angiogenesis
  • Neurite dynamics
  • Cell invasion & migration
  • 3D Spheroid Formation & growth in ultra-low attachment plates
  • Apoptosis and Cytotoxicity
  • Proliferation
  • Fibrosis Modelling
  • Receptor internalisation
  • Cell cycle analysis
  • Rapid and reproducible quantitative data output for evaluation of compound effects on cell health and target parameters
  • A number of established endpoint markers or utilise our knowledge to select suitable markers for your chosen target

Examples include:

Mitochondrial Depolarisation:

Neurite Dynamics:

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