Inflammation and Immunology
Sygnature Discovery have developed a broad understanding of inflammatory and immune mechanisms and pathways that represent major components of many disease pathologies. Our expert scientists are able to respond in a flexible manner to develop meaningful and efficient assay cascades to support your research project.
Our bioscientists have significant experience in developing in vitro assays to assess various inflammation and immunological biomarkers, processes, and endpoints. We have an in-house Blood Donor Panel allowing ready access to whole blood, PBMC or isolated key immune and inflammatory cells from healthy donors. In addition, we can access whole blood and blood-derived cells from appropriate patients through contacts with local hospitals, universities and specialist suppliers, obtaining either fresh or cryopreserved samples.
Integrated projects involving our co-located Chemistry, DMPK, Computational Chemistry and Bioscience teams allow us to work with our clients to efficiently deliver strong candidate drugs; Optimising Leads to provide excellent ADMET properties appropriate to the target indication, whether topical or systemic.
We have a range of assays readily available to study key inflammatory and immune processes including:
- Proliferation, Chemotaxis, phagocytosis, endocytosis, mediator release (e.g. ELISA, AlphaLISA, Luminex).
- Changes in Intracellular Signalling molecules, such as calcium and phosphoprotein levels.
- Expertise in a number of biochemical and cellular platforms that can be used to study inflammatory and immune target pharmacology allows us to develop Bespoke Assays to suit your requirements:
- Biophysical Techniques to study protein and/or epigenetic targets,
- qPCR to study Gene Expression changes
Imaging platforms to study both real-time live and/or fixed cells (Flow Cytometry, IncuCyte Zoom, Image Express) which can be utilised to investigate key cellular events.