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Biochemical Assays

Our bioscientists are well versed in the development and maintenance of robust tailored assays spanning a plethora of enzyme targets.

Sourced, either off the shelf from commercial vendors or developed in-house to fit our clients’ requirements, our experienced scientists provide high-quality biochemical assays and informed data interpretation across the discovery pipeline.

We can offer a variety of scalable enzyme activity assays (product formation and detection) and Binding Assays using industry-standard instrumentation platforms across multiple enzyme classes

Enzyme Activity Assays:

  • Kinase
  • Methyltransferase
  • Demethylase
  • Protease
  • PDE
  • Phosphatase
  • DUB
  • Others

Binding Assays:

  • Radiolabel binding
  • Fluorescence Polarisation (FP) binding assays
  • Nuclear Hormone
  • Ion Channels
  • Others


  • BRET (bioluminescence resonance energy transfer), NanoBRET™, HTRF (homogeneous time-resolved fluorescence).
  • Fluorescence Intensity, Absorbance, Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET), LC-MS (liquid chromatography–mass spectrometry).
  • AlphaScreen® (amplified luminescent proximity homogeneous assay).

Biochemical Assays Services


Our scientists are well versed in enzymology being experts at optimising activity, characterising enzyme kinetics and determining mechanisms of inhibition, enabling the delivery of high quality data to inform your discovery program.


Binding Assays

The bioscientists at Sygnature Discovery have extensive experience in developing a wide range of binding assay in a variety of formats, ensuring your project has the tailored assay solution it requires.