Biochemical Assays

Our bioscientists are well versed in the development and maintenance of robust tailored assays spanning a plethora of enzyme targets.

Sourced, either off the shelf from commercial vendors or developed in-house to fit our clients’ requirements, our experienced scientists provide high-quality biochemical assays and informed data interpretation across the discovery pipeline.

We can offer a variety of scalable enzyme activity assays (product formation and detection) and Binding Assays using industry-standard instrumentation platforms across multiple enzyme classes.

Enzyme Activity Assays:

  • Kinase
  • Methyltransferase
  • Demethylase
  • Protease
  • PDE
  • Phosphatase
  • DUB
  • Others

Binding Biochemical Assays:

  • Radiolabel binding
  • Fluorescence Polarisation (FP) binding assays
  • Nuclear Hormone
  • Ion Channels
  • Others


  • BRET (bioluminescence resonance energy transfer), NanoBRET™, HTRF (homogeneous time-resolved fluorescence).
  • Fluorescence Intensity, Absorbance, Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET), LC-MS (liquid chromatography–mass spectrometry).
  • AlphaScreen® (amplified luminescent proximity homogeneous assay).

Biochemical Assays Services


Rely on our expert scientists proficient in enzymology, skilled in optimising activity, characterising enzyme kinetics, and elucidating mechanisms of inhibition. Trust us to provide high-quality data that informs your discovery program effectively.


Binding Assays

Maximise the potential of your project with Sygnature Discovery's renowned team of bioscientists. Drawing on their extensive experience, we specialise in developing a wide range of binding assays in various formats. Trust us to provide the tailored assay solution that precisely matches your project's unique needs.