Bespoke Assay Development
At Sygnature Discovery our bioscientists have the specialism and depth of knowledge to develop novel assay solutions for the most challenging of projects. This is complemented by extensive experience of supporting projects across the discovery pipeline, ensuring timely delivery of a robust scalable assay that is fit for purpose and that can be fully integrated into a streamlined Screening Cascade.
Our scientists can recommend novel approaches and develop bespoke solutions for your application when commercially-available assays are unavailable. These validated assays can be created ‘from scratch’, developed in collaboration with commercial suppliers or transferred from your laboratory, with any necessary optimisation made so they are suitable to meet your projects demands. Our scientists undertake a careful iterative evaluation of potential parameters that may affect your assay, taking necessary steps to ensure your assay is always capable of meeting project demands. Sygnature’s approach to bespoke assay development is further complemented by robust data analysis that we strongly believe is critical in delivering high-quality interpretable information to advance your discovery programs.
At Sygnature Discovery we use several guiding principles that are considered via client engagement when developing a bespoke biological assay-
- Understanding the assay purpose
- Identifying different assay formats
- Developing or sourcing optimal assay reagents
- Monitoring assay performance
- Options for automation and scaling
- Quality control via statistical validation
By adopting this approach, we can address one of the most significant key challenges in drug discovery, namely time and cost.
We ensure that our bespoke assays offer-
- Increased relevance of biological information via comprehensive validation of reagents and assays
- Efficient experimental design to maximise value-adding information
Your novel compounds can be assessed for their potency, efficacy, kinetics, Mechanism-of-Action, Selectivity, species cross-over, and more, which we endeavour to assess within a physiologically-relevant context.
Example developing a custom assay to support hit finding activities for novel targets:
- A bespoke PPI assay was developed to identify specific site-directed inhibitors
- There were no commercially available reagents
- We recommended AlphaScreen because of its sensitivity
We undertake optimisation of assay formats to improve assay throughput, reduce time and costs for our clients.
If you are unable to find an assay that fits your needs on our website, then please contact our team to discuss other custom assay options we can offer to meet your requirements.