Physical dependence and withdrawal
Withdrawal symptoms, indicative of physical dependence can occur following the abrupt cessation of sub-chronic dosing of a CNS active molecule. Sygnature has developed a model for evaluating withdrawal-induced physical dependence in rats and validated it using both opiates and benzodiazepines. All physical dependence and withdrawal studies can be performed to GLP to meet regulatory requirements.
Cessation of dosing following 28-days morphine administration results in clear withdrawal signs in the rat
Significant differences vs. vehicle: *p<0.05, **p<0.01 and ***p<0.001.