Assessing Lypoprotectant Effect on Particle Size of Liposomal Systems

Liposomes are a versatile drug deliver platform, capable of encapsulating a broad range of therapeutic agents, including those requiring targeted delivery, exhibiting poor solubility or associated toxicity risks. However, when stored in liquid suspension, liposomes face stability challenges such as aggregation, fusion and drug leakage over time.

This study investigates freeze-drying (lyophilisation) as a strategy to overcome these limitations and explores the role of different lyoprotectants in preserving liposome integrity and particle size during the process. Two lyoprotectants, trehalose and hydroxypropyl beat-cyclodextrin (HPβCD), were evaluated to determine the effectiveness in stabilising freeze-dried liposomal formulations.

Our findings highlight the critical importance of lyoprotectant selection in developing stable, freeze-dried liposomal formulations. These results emphasise the need for tailored formulation strategies rather than a one-size-fits-all approach, supporting the development of robust drug delivery solutions for complex therapeutics.