Dexamethasone and Enalaprilat but not SB525334 exhibit anti-inflammatory properties in precision-cut kidney slices (PCKS) obtained from mice with Adriamycin-induced nephropathy

Precision-cut kidney slices (PCKS) serve as a vital intermediary stage bridging the gap between in vitro and in vivo testing. When PCKS are derived from rodents exposed to adriamycin, they manifest distinct pathological features linked to glomerular and tubular injury, inflammation, and eventual fibrosis.

This study sought to assess the impact of dexamethasone, enalaprilat, and SB525334 on inflammation and fibrosis within PCKS obtained from kidney slices of adriamycin-treated mice. The results demonstrated that dexamethasone and enalaprilat were effective in reducing inflammation and, in the case of dexamethasone, mitigating fibrosis. These effects contributed to the preservation of the kidney’s functional unit.

Conversely, SB52334 primarily affected fibrosis, and its impact on overall kidney health remained inconclusive.

Collectively, the findings underscore the critical role of inflammation in adriamycin-induced nephropathy and highlight PCKS as a reliable platform for evaluating novel anti-inflammatory and anti-fibrotic therapies.