Determination of the profile of a range of psychedelics for 5-HT1A, 5-HT2A and 5-HT2C receptors in rodent brain tissue

Receptor binding assays utilise ligands labelled with radioisotopes such as tritium ([³H]) to determine the pharmacology of non-radiolabelled compounds at a receptor of interest. Competition assays determine the affinity of a ligand for a receptor by displacing a known concentration of a radioligand. At Sygnature Discovery, we have recently validated binding assays to determine the affinity of a range of psychedelics for 5-HT_1A, 5-HT_2A and 5-HT_2c receptors using [³H]8-OH-DPAT, [³H]Cimbi-36 and [³H]Mesulergine, respectively, in rodent brain. This enables us to compare the receptor binding profiles of a range of psychedelic and non-psychedelic compounds.