Comparison of (±)MDMA, fluoxetine, and dexfenfluramine to inhibit uptake and/or stimulate release of [3H]5-HT in rat frontal cortical synaptosomes
Uptake and release assays are functional in vitro techniques used to investigate the ability of compounds to either inhibit reuptake or induce release of neurotransmitters through the monoamine transporters. The serotonin (5-HT) transporter is largely responsible for regulating serotonergic neurotransmission within the central nervous system (CNS) and therefore is the principal target for many medications in the treatment of a range of psychiatric disorders such as anxiety, PTSD and depression. The aim of this study was to assess the ability of (±)MDMA, dexfenfluramine and fluoxetine to inhibit uptake and stimulate release of [3H]5-HT into/from rat frontal cortical synaptosomes.