Accelerating Reaction Development Through High-Throughput Experimentation

High-Throughput Experimentation for Faster Route Progression

Identifying robust reaction conditions can be one of the most time-consuming stages of synthetic chemistry. High-throughput experimentation (HTE) enables scientists to rapidly evaluate multiple reaction parameters in parallel, accelerating route development while minimising material consumption.

At Sygnature Discovery, our dedicated HTE team helps chemists quickly identify suitable reaction conditions to advance projects more efficiently. Using ChemBeads-enabled screening technologies, reactions can be assessed on just 2-3 µmol of substrate per well, allowing comprehensive reaction screening with minimal material requirements. Standard screening plates are designed using a combination of literature precedents and extensive internal expertise, with flexible formats available to meet project-specific needs.

A Flexible High-Throughput Screening Platform

Our platform supports 24-, 48- and 96-well screening formats and can be tailored to client requirements by adding or removing reaction conditions as needed. Scientists can rapidly assess catalysts, ligands, solvents, bases, additives and other reaction variables to identify promising conditions in a fraction of the time required for traditional sequential experimentation.

The platform supports more than 15 validated reaction types and has been successfully applied across over 60 client projects. Alongside condition discovery, HTE can also be used for iterative optimisation studies focused on improving yields, reducing impurities, enhancing selectivity or lowering manufacturing costs.

Supporting Direct-to-Biology Strategies

High-throughput experimentation is frequently used to enable direct-to-biology (D2B) workflows, allowing rapid assessment of reaction conditions in biologically relevant settings. By combining automated reaction set-up, efficient analytical workflows and rapid data reporting, chemists can make informed decisions and move projects forward faster.

Key Benefits of Sygnature Discovery’s HTE Platform

Rapid Reaction Screening

• Evaluate multiple reaction parameters simultaneously to significantly reduce experimental design cycles and accelerate decision-making.

Diverse Chemical Capabilities

• Access a broad range of validated reaction types capable of addressing complex synthetic chemistry challenges.

Scalable Hit Validation

• Promising conditions identified during screening are validated at larger scale to ensure reproducibility and reliability before progression.

Continuous Platform Evolution

• Our workflows continuously incorporate new scientific literature, emerging technologies and internal learnings to deliver optimal outcomes for clients.

Download the Poster

Explore real-world examples demonstrating how high-throughput experimentation can accelerate reaction optimisation, support direct-to-biology workflows and overcome complex synthetic challenges.