Prep SFC at Scale: 10 g to 100 g Purifications

Scenario A – 10 g

• Prep column: 30 mm diameter
• Loading: ~23,33 mg/injection, approx. 689 mg/h
• Run time: 14.5 h
• Smooth transition from analytical to prep column

Scenario B – 15 g

• Prep column: 30 mm, 100 mL/min flow
• Loading: 100 mg/injection, approx. 909 mg/h
• Total runtime: 16.5 h
• Efficient handling of high-throughput purification

Scenario C – 100 g

• Analytical column: 4.6 mm, 4 mL/min flow
• Prep column: 50 mm, 250 mL/min flow
• Loading: 40 mg/injection, approx. 757 mg/h
• Total runtime: 132 h
• Efficient handling of high-throughput purification

These projects illustrate how we scale purification without disrupting the discovery-to-preclinical continuum, delivering large quantities of high-purity compound, cost-effectively.