{"id":3082,"date":"2021-02-11T14:24:10","date_gmt":"2021-02-11T14:24:10","guid":{"rendered":"https:\/\/www.sygnaturediscovery.com\/technical-note\/aqueous-solubility-turbidimetric-kinetic-thermodynamic\/"},"modified":"2021-02-11T14:24:10","modified_gmt":"2021-02-11T14:24:10","slug":"aqueous-solubility-turbidimetric-kinetic-thermodynamic","status":"publish","type":"technical-note","link":"https:\/\/www.sygnaturediscovery.com\/fr\/technical-note\/aqueous-solubility-turbidimetric-kinetic-thermodynamic\/","title":{"rendered":"Aqueous solubility \u2013 turbidimetric \/ kinetic \u2013 thermodynamic"},"content":{"rendered":"

Low solubility can be a major obstacle during drug discovery and development, as low solubility can impact the generation of quality\u00a0in vitro<\/em>\u00a0DMPK or biology data, can cause issues in the generation of an appropriate formulation for\u00a0in vivo<\/em>\u00a0pharmacokinetic studies or can cause issues with drug absorption following oral dosing\u00a0in vivo<\/em>. If the solubility is known, then solutions can easily be made to the correct concentrations.<\/p>\n

Solubility is defined as the maximum amount of a chemical (the solute) that dissolves in a given volume of a solvent. Although solubility is determined by a compound\u2019s physicochemical properties such as lipophilicity (logD7.4<\/sub>), acid dissociation constant (pKa), the number of hydrogen bond donors and acceptors and its polar surface area (PSA),\u00a0in silico\u00a0<\/em>predictions are often not very accurate.<\/p>\n

For drugs, aqueous solubility is a fundamentally important property that affects not only the potential for drug absorption after oral administration and the ability to administer the drug parenterally, but also the ease of manipulation and testing in the laboratory and during manufacture. (Williams et al.,2013)<\/p>\n

Potential issues with poorly soluble compounds include:<\/p>\n