{"id":3024,"date":"2018-03-07T12:35:08","date_gmt":"2018-03-07T12:35:08","guid":{"rendered":"https:\/\/www.sygnaturediscovery.com\/technical-note\/microsomal-metabolic-stability\/"},"modified":"2018-03-07T12:35:08","modified_gmt":"2018-03-07T12:35:08","slug":"microsomal-metabolic-stability","status":"publish","type":"technical-note","link":"https:\/\/www.sygnaturediscovery.com\/fr\/technical-note\/microsomal-metabolic-stability\/","title":{"rendered":"Microsomal Clearance and Stability"},"content":{"rendered":"

The liver is the major site of drug metabolism in the body, with well over 50% of marketed drugs being eliminated via hepatic mediated metabolism. Hence, measurement of the rate of clearance and the identity of the metabolites of a drug is important. In addition, in support of projects, the rate of clearance values can be used to rank compounds and to predict\u00a0in vivo<\/em>\u00a0hepatic clearance values.\u00a0Microsomes from a variety of species and tissues can be used to understand inter-species differences and non-hepatic metabolism.<\/p>\n

Sygnature\u2019s standard microsomal metabolic stability assay utilises the Phase I cofactor, NADPH. We can also offer a combined Phase I\/Phase II assay, utilising NADPH and UDPGA to determine both Phase 1 and Phase 2 (UDP-glucuronosyltransferase (UGT)) metabolism. For the latter, these are accessed by pre-incubating the microsomes with the pore-forming agent, alamethicin.<\/p>\n

Sygnature\u2019s microsomal stability assay monitors the disappearance of a compound in the presence and absence of cofactor. All assays are validated by the inclusion of up to 3 species-specific control compounds.\u00a0Data output consists of mean intrinsic clearance and half-life measurements.<\/p>\n","protected":false},"excerpt":{"rendered":"

Discover the significance of hepatic drug metabolism through microsomal clearance studies. Sygnature offers NADPH-based Phase I assays and combined Phase I\/Phase II assays for comprehensive metabolism insights. Gain compound ranking and predict in vivo hepatic clearance. Validated with species-specific controls, get accurate mean intrinsic clearance and half-life measurements.<\/p>\n","protected":false},"featured_media":0,"template":"","category":[1],"resource_tag":[],"class_list":["post-3024","technical-note","type-technical-note","status-publish","hentry","category-uncategorized"],"acf":[],"yoast_head":"\nMicrosomal Clearance & Stability - Sygnature Discovery<\/title>\n<meta name=\"description\" content=\"Enhancing drug metabolism analysis: Sygnature's refined microsomal assays offer accurate insights into clearance rates and metabolic pathways.\" \/>\n<meta name=\"robots\" content=\"noindex, follow\" \/>\n<meta property=\"og:locale\" content=\"fr_CA\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Microsomal Clearance & Stability - Sygnature Discovery\" \/>\n<meta property=\"og:description\" content=\"Enhancing drug metabolism analysis: Sygnature's refined microsomal assays offer accurate insights into clearance rates and metabolic pathways.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.sygnaturediscovery.com\/fr\/technical-note\/microsomal-metabolic-stability\/\" \/>\n<meta property=\"og:site_name\" content=\"Sygnature\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2026\/02\/sygnature-hit-to-lead-team-collaboration-scaled.webp\" \/>\n\t<meta property=\"og:image:width\" content=\"2560\" \/>\n\t<meta property=\"og:image:height\" content=\"1707\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/webp\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Estimation du temps de lecture\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/technical-note\\\/microsomal-metabolic-stability\\\/\",\"url\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/technical-note\\\/microsomal-metabolic-stability\\\/\",\"name\":\"Microsomal Clearance & Stability - 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