The liver is the major site of drug metabolism in the body, with well over 50% of marketed drugs eliminated via hepatic mediated metabolism. Hence, measurement of the rate of clearance and the identity of the metabolites of a drug is immensely important.<\/p>\n
In addition, in support of projects, the rate of clearance values can be used to rank compounds and to predict\u00a0in vivo<\/em>\u00a0hepatic clearance values. Hepatocytes from a variety of species can be used to understand inter-species differences and are essential when generating Dose to Human predictions. Unlike microsomal stability assays, both Phase I and Phase II metabolism can be tracked, and the rate of clearance and their metabolites formed are more likely to reflect the\u00a0in vivo<\/em>\u00a0situation.<\/p>\n Sygnature\u2019s Hepatocyte Stability assay monitors the disappearance of a substrate in the presence and absence of hepatocytes. All assays are validated by the inclusion of up to 3 species-specific positive control compounds. Data output consists of mean intrinsic clearance (Clint<\/sub>) and half-life (t1\/2<\/sub>) measurements.<\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"featured_media":0,"template":"","category":[1],"resource_tag":[],"class_list":["post-3019","technical-note","type-technical-note","status-publish","hentry","category-uncategorized"],"acf":[],"yoast_head":"\n