{"id":4231,"date":"2025-11-20T10:10:41","date_gmt":"2025-11-20T10:10:41","guid":{"rendered":"https:\/\/www.sygnaturediscovery.com\/poster\/il-4-il-3-translation-platform\/"},"modified":"2026-01-22T08:43:32","modified_gmt":"2026-01-22T08:43:32","slug":"il-4-il-3-translation-platform","status":"publish","type":"poster","link":"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/il-4-il-3-translation-platform\/","title":{"rendered":"Development of an IL-4\/IL-13:IL-4R\u03b1 in vitro to in vivo Translational Platform in a Model of Respiratory Disease."},"content":{"rendered":"\n

Type 2 inflammation drives many chronic respiratory diseases, include asthma and COPD, with IL-4 and IL-13 signalling through IL-4R\u03b1 and JAK\/STAT6 as central pathway nodes. While the success of dupilumab clinically validates IL-4R\u03b1 as a target, there remains a need for efficient, translational assays to discover and characterize next-generation modulators of this pathway. In collaboration with Numerion, Sygnature Discovery has established an integrated in vitro <\/em>to in vivo <\/em>platforms that links IL-4\/IL-13:IL-4R\u03b1 target engagement and signalling with downstream chemokine readouts in a respiratory disease-relevant mouse model.<\/p>\n\n\n\n

Using surface plasmon resonance (SPR), we demonstrated high-affinity binding of IL-4 and dupilumab to IL-4R\u03b1, and confirmed disruption of the IL-4:IL-4R\u03b1 protein-protein interactions in a sandwich ELISA format. Cellular assays in BEAS-2B lung epithelial cells and mouse splenocytes then captured pathway activation (pSTAT6) and downstream chemokine release (Eotaxin-1), enabling us to benchmark the clinically validated IL-4R\u03b1 antibody dupilumab and the JAK inhibitor tofacitinib. Finally, an intranasal IL-13 Balb\/c model provided in vivo <\/em>confirmation that Tofacitinib reduces lung Eotaxin-1 and Eotaxin-2 in a dose-dependent manner with corresponding plasma exposure, complementing a medium-throughput translational cascade suitable for profiling novel IL-4R\u03b1 pathway therapeutics in respiratory and other Th2-driven indications.<\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"featured_media":3010,"template":"","category":[711,681,752,685,688],"class_list":["post-4231","poster","type-poster","status-publish","has-post-thumbnail","hentry","category-biophysical-assays","category-bioscience","category-ii","category-in-vivo-pharmacology","category-therapeutic-areas"],"acf":[],"yoast_head":"\nDevelopment of an IL-4\/IL-13:IL-4R\u03b1 in vitro to in vivo Translational Platform in a Model of Respiratory Disease.<\/title>\n<meta name=\"description\" content=\"Discover Sygnature\u2019s IL-4\/IL-13:IL-4R\u03b1 in vitro-to-in vivo 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