{"id":4229,"date":"2025-11-18T08:00:24","date_gmt":"2025-11-18T08:00:24","guid":{"rendered":"https:\/\/www.sygnaturediscovery.com\/poster\/electrophysiological-characterization-of-tmem175-variants\/"},"modified":"2026-04-03T14:52:47","modified_gmt":"2026-04-03T14:52:47","slug":"electrophysiological-characterization-of-tmem175-variants","status":"publish","type":"poster","link":"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/electrophysiological-characterization-of-tmem175-variants\/","title":{"rendered":"Electrophysiological Characterization of Functional and Pharmacological Effects of TMEM175 Wild Type, Gain-of-Function and Loss-of-Function Lysosomal Coding Variants"},"content":{"rendered":"

TMEM175 is a proton-activated lysosomal ion channel that regulates lysosomal pH and autophagy, and mutations in this channel have been linked to Parkinson’s disease risk. Sygnature Discovery developed recombinant HEK-293 cell lines expressing TMEM175 wild type (WT), gain-of-function (GoF) and loss-of-function (LoF) variants to examine proton and ceasium conductance using the SycroPatch 384i automated electrophysiology platform. Electrophysiological characterization included pH sensitivity, proton-activated and caesium currents, and pharmacological modulation using a known activator (DCPIB) and inhibitor (4-AP). Results revealed expected pH-dependent channel responses, variant-specific shifts in sensitivity, and clear potency changes under different pH conditions. This high-throughput assay enables rapid profiling of TMEM175 modulators and provides a translational platform to explore the role of lysosomal ion channel function in Parkinson’s disease.<\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"featured_media":3007,"template":"","category":[681,709,707,746,753,687,688],"class_list":["post-4229","poster","type-poster","status-publish","has-post-thumbnail","hentry","category-bioscience","category-cell-based-assays","category-electrophysiology","category-ion-channels","category-neuroscience","category-target-classes","category-therapeutic-areas"],"acf":[],"yoast_head":"\nElectrophysiological Characterization of TMEM175 Variants<\/title>\n<meta name=\"description\" content=\"Sygnature Discovery\u2019s automated electrophysiology study of TMEM175 variants reveals pH-dependent conductance and pharmacological responses.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, 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