{"id":4195,"date":"2025-10-21T07:00:29","date_gmt":"2025-10-21T07:00:29","guid":{"rendered":"https:\/\/www.sygnaturediscovery.com\/poster\/gpcr-activation-nanobit-assay-ligand-screening\/"},"modified":"2026-01-22T13:18:02","modified_gmt":"2026-01-22T13:18:02","slug":"gpcr-activation-nanobit-assay-ligand-screening","status":"publish","type":"poster","link":"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/gpcr-activation-nanobit-assay-ligand-screening\/","title":{"rendered":"Quantifying GPCR Activation with NanoBiT Assay for Ligand Screening"},"content":{"rendered":"<p><strong>A versatile NanoBiT assay for ligand screening<\/strong><\/p>\n<p>GPCRs remain key therapeutic targets, yet many &#8211; including receptors with complex G-protein coupling, such as those linked to G\u03b112\/13- are difficult to study using conventional functional assays.<\/p>\n<p>Sygnature Discovery has developed a NanoBiT-based biosensor platform that quantifies GPCR activation through G\u03b2\u03b3-GRK interactions, providing a proximal, live-cell readout of receptor engagement.<\/p>\n<p>The assay delivers robust, high-throughput performance, compatible with automation and orthogonal validation using chimeric G-protein calcium flux assays, enabling confident discrimination between agonists, partial agonists and antagonists, and is adaptable to a wide range of GPCR families.<\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"featured_media":2958,"template":"","category":[681,709,747,687],"class_list":["post-4195","poster","type-poster","status-publish","has-post-thumbnail","hentry","category-bioscience","category-cell-based-assays","category-gpcrs","category-target-classes"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Quantifying GPCR Activation with NanoBiT Assay for Ligand Screening<\/title>\n<meta name=\"description\" content=\"Discover how Sygnature 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