{"id":4156,"date":"2025-05-21T07:00:34","date_gmt":"2025-05-21T07:00:34","guid":{"rendered":"https:\/\/www.sygnaturediscovery.com\/poster\/high-content-screen-nlrp1-inflammasome-modulators\/"},"modified":"2026-01-22T14:04:29","modified_gmt":"2026-01-22T14:04:29","slug":"high-content-screen-nlrp1-inflammasome-modulators","status":"publish","type":"poster","link":"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/high-content-screen-nlrp1-inflammasome-modulators\/","title":{"rendered":"A &lsquo;Speckulative&rsquo; Screen: High-Content MTS Against NLRP1 Mediated Inflammasome Formation"},"content":{"rendered":"<p><span style=\"font-size: 14pt;\">Sygnature Discovery presents a poster exploring the development of a robust high-content screening platform to uncover small molecule modulators of NLRP1-mediated inflammasome formation. This fixed-endpoint, image-based assay uses ASC::GFP-labelled cells to visualise inflammasome speck formation and assess the impact of 5,120 diverse compounds from Sygnature\u2019s LeadFinder library to identify potential modulators of NLRP1 mediated inflammasome formation as a key pattern recognition receptor.<\/span><\/p>\n<h2><span style=\"font-size: 14pt;\"><strong>Our NLRP1 Inflammasome Screening Highlights<\/strong><\/span><\/h2>\n<p>A fixed-endpoint assay was established to identify potential modulators of\u00a0 inflammasome formation.<\/p>\n<ul>\n<li data-start=\"489\" data-end=\"576\"><span style=\"font-size: 12pt;\">High-content imaging to track speck formation as a readout of inflammasome activation<\/span><\/li>\n<li data-start=\"579\" data-end=\"637\"><span style=\"font-size: 12pt;\">5120-compound primary screen yielding 70+ modulating hits<\/span><\/li>\n<li data-start=\"640\" data-end=\"709\"><span style=\"font-size: 12pt;\">Potency profiling revealed multiple novel activators and inhibitors<\/span><\/li>\n<\/ul>\n<h2 data-start=\"0\" data-end=\"61\"><span style=\"font-size: 14pt;\"><strong data-start=\"0\" data-end=\"61\">Advancing Innate Immunity Research Through HTS Innovation<\/strong><\/span><\/h2>\n<p data-start=\"63\" data-end=\"373\" data-is-last-node=\"\" data-is-only-node=\"\">This study demonstrates how NLRP1 inflammasome screening, more specifically how automated high-content imaging and Sygnature\u2019s integrated screening workflow can be effectively applied to innate immunity targets, enabling early-stage identification and validation of promising NLRP1 pathway modulators in drug discovery.<\/p>\n<p><strong>Download the poster to explore how Sygnature Discovery\u2019s high-throughput screening platform is used for NLRP1 inflammasome formation.<\/strong><\/p>\n<p><span style=\"font-size: 12pt;\">Sygnature Discovery supports complex <a href=\"https:\/\/www.sygnaturediscovery.com\/drug-discovery\/bioscience\/ion-channel-screening\/\" target=\"_blank\" rel=\"noopener\">cell-based screening<\/a> with high-content imaging, assay development, compound profiling, and target validation to drive drug discovery forward with the best chance at a successful <a href=\"https:\/\/www.sygnaturediscovery.com\/drug-discovery\/integrated-drug-discovery\/hit-identification\/\" target=\"_blank\" rel=\"noopener\">hit finding<\/a> and validation stage.<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"featured_media":2902,"template":"","category":[706,681,708,752],"class_list":["post-4156","poster","type-poster","status-publish","has-post-thumbnail","hentry","category-assay-development","category-bioscience","category-high-throughput-screening","category-ii"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.5 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>High-Content NLRP1 Inflammasome Screening<\/title>\n<meta name=\"description\" content=\"Discovery how 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