{"id":4086,"date":"2024-08-16T12:04:21","date_gmt":"2024-08-16T12:04:21","guid":{"rendered":"https:\/\/www.sygnaturediscovery.com\/poster\/discovery-of-novel-ligase-ligands-using-affinity-selection-mass-spectrometry\/"},"modified":"2026-01-22T15:18:55","modified_gmt":"2026-01-22T15:18:55","slug":"discovery-of-novel-ligase-ligands-using-affinity-selection-mass-spectrometry","status":"publish","type":"poster","link":"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/discovery-of-novel-ligase-ligands-using-affinity-selection-mass-spectrometry\/","title":{"rendered":"Discovery of Novel Ligase Ligands Using Affinity Selection Mass Spectrometry"},"content":{"rendered":"<p>Affinity selection mass spectrometry (ASMS) is a label-free binding method that enables the identification of novel ligands for drug discovery. Since ASMS is agnostic of the binding mechanism, it is ideally suited to discovering ligands for targeted protein degradation (TPD).<\/p>\n<p>We performed a screen of our LeadFinder library of 150K lead-like compounds to identify compounds binding to the VHL complex, VCB, that could serve as starting points for <a href=\"https:\/\/www.sygnaturediscovery.com\/drug-discovery\/integrated-drug-discovery\/targeted-protein-degradation\/\">TPD<\/a> programmes. Using our 2DLC-TOF system we performed an online size exclusion chromatography (SEC) ASMS method based on the automated ligand identification system (ALIS).<\/p>\n<p>We identified novel binders from the pooled screen, subsequently confirmed in an ASMS retest of singletons. This work highlights the potential of ASMS in advancing drug discovery efforts, particularly for TPD applications, by providing a reliable and efficient approach to discovering high-affinity ligands.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>In this poster, we demonstrate ASMS screening for novel VHL ligands as starting points for TPD programs. Download for more insights into how ASMS advances drug discovery by efficiently identifying high-affinity ligands.<\/p>\n","protected":false},"featured_media":2793,"template":"","category":[756,681,708,690,686,743],"class_list":["post-4086","poster","type-poster","status-publish","has-post-thumbnail","hentry","category-asms","category-bioscience","category-high-throughput-screening","category-hit-identification","category-modalities","category-tpd"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.5 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Affinity Selection Mass Spectrometry (ASMS) for VHL Ligands<\/title>\n<meta name=\"description\" content=\"A 150K-compound LeadFinder library screen against the VHL complex (VCB), with novel binders confirmed in singleton retests for TPD programs. Learn more.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/discovery-of-novel-ligase-ligands-using-affinity-selection-mass-spectrometry\/\" \/>\n<meta property=\"og:locale\" content=\"fr_CA\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Affinity Selection Mass Spectrometry (ASMS) for VHL Ligands\" \/>\n<meta property=\"og:description\" content=\"A 150K-compound LeadFinder library screen against the VHL complex (VCB), with novel binders confirmed in singleton retests for TPD programs. 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