{"id":4086,"date":"2024-08-16T12:04:21","date_gmt":"2024-08-16T12:04:21","guid":{"rendered":"https:\/\/www.sygnaturediscovery.com\/poster\/discovery-of-novel-ligase-ligands-using-affinity-selection-mass-spectrometry\/"},"modified":"2026-01-22T15:18:55","modified_gmt":"2026-01-22T15:18:55","slug":"discovery-of-novel-ligase-ligands-using-affinity-selection-mass-spectrometry","status":"publish","type":"poster","link":"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/discovery-of-novel-ligase-ligands-using-affinity-selection-mass-spectrometry\/","title":{"rendered":"Discovery of Novel Ligase Ligands Using Affinity Selection Mass Spectrometry"},"content":{"rendered":"

Affinity selection mass spectrometry (ASMS) is a label-free binding method that enables the identification of novel ligands for drug discovery. Since ASMS is agnostic of the binding mechanism, it is ideally suited to discovering ligands for targeted protein degradation (TPD).<\/p>\n

We performed a screen of our LeadFinder library of 150K lead-like compounds to identify compounds binding to the VHL complex, VCB, that could serve as starting points for TPD<\/a> programmes. Using our 2DLC-TOF system we performed an online size exclusion chromatography (SEC) ASMS method based on the automated ligand identification system (ALIS).<\/p>\n

We identified novel binders from the pooled screen, subsequently confirmed in an ASMS retest of singletons. This work highlights the potential of ASMS in advancing drug discovery efforts, particularly for TPD applications, by providing a reliable and efficient approach to discovering high-affinity ligands.<\/p>\n","protected":false},"excerpt":{"rendered":"

In this poster, we demonstrate ASMS screening for novel VHL ligands as starting points for TPD programs. Download for more insights into how ASMS advances drug discovery by efficiently identifying high-affinity ligands.<\/p>\n","protected":false},"featured_media":2793,"template":"","category":[756,681,708,690,686,743],"class_list":["post-4086","poster","type-poster","status-publish","has-post-thumbnail","hentry","category-asms","category-bioscience","category-high-throughput-screening","category-hit-identification","category-modalities","category-tpd"],"acf":[],"yoast_head":"\nDiscovery of Novel Ligase Ligands Using Affinity Selection Mass Spectrometry - Sygnature<\/title>\n<meta name=\"description\" content=\"In this poster, we demonstrate ASMS screening for novel VHL ligands in TPD programs. Download for more insights.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/discovery-of-novel-ligase-ligands-using-affinity-selection-mass-spectrometry\/\" \/>\n<meta property=\"og:locale\" content=\"fr_CA\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Discovery of Novel Ligase Ligands Using Affinity Selection Mass Spectrometry - Sygnature\" \/>\n<meta property=\"og:description\" content=\"In this poster, we demonstrate ASMS screening for novel VHL ligands in TPD programs. Download for more insights.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/discovery-of-novel-ligase-ligands-using-affinity-selection-mass-spectrometry\/\" \/>\n<meta property=\"og:site_name\" content=\"Sygnature\" \/>\n<meta property=\"article:modified_time\" content=\"2026-01-22T15:18:55+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/11\/Discovery-of-novel-ligase-ligands-using-asms.png\" \/>\n\t<meta property=\"og:image:width\" content=\"628\" \/>\n\t<meta property=\"og:image:height\" content=\"807\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Estimation du temps de lecture\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/poster\\\/discovery-of-novel-ligase-ligands-using-affinity-selection-mass-spectrometry\\\/\",\"url\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/poster\\\/discovery-of-novel-ligase-ligands-using-affinity-selection-mass-spectrometry\\\/\",\"name\":\"Discovery of Novel Ligase Ligands Using Affinity Selection Mass Spectrometry - Sygnature\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/poster\\\/discovery-of-novel-ligase-ligands-using-affinity-selection-mass-spectrometry\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/poster\\\/discovery-of-novel-ligase-ligands-using-affinity-selection-mass-spectrometry\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/wp-content\\\/uploads\\\/2025\\\/11\\\/Discovery-of-novel-ligase-ligands-using-asms.png\",\"datePublished\":\"2024-08-16T12:04:21+00:00\",\"dateModified\":\"2026-01-22T15:18:55+00:00\",\"description\":\"In this poster, we demonstrate ASMS screening for novel VHL ligands in TPD programs. Download for more insights.\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/poster\\\/discovery-of-novel-ligase-ligands-using-affinity-selection-mass-spectrometry\\\/#breadcrumb\"},\"inLanguage\":\"fr-CA\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/poster\\\/discovery-of-novel-ligase-ligands-using-affinity-selection-mass-spectrometry\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"fr-CA\",\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/poster\\\/discovery-of-novel-ligase-ligands-using-affinity-selection-mass-spectrometry\\\/#primaryimage\",\"url\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/wp-content\\\/uploads\\\/2025\\\/11\\\/Discovery-of-novel-ligase-ligands-using-asms.png\",\"contentUrl\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/wp-content\\\/uploads\\\/2025\\\/11\\\/Discovery-of-novel-ligase-ligands-using-asms.png\",\"width\":628,\"height\":807},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/poster\\\/discovery-of-novel-ligase-ligands-using-affinity-selection-mass-spectrometry\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/page-daccueil\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Discovery of Novel Ligase Ligands Using Affinity Selection Mass Spectrometry\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/#website\",\"url\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/\",\"name\":\"Sygnature Discovery\",\"description\":\"\",\"publisher\":{\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/#organization\"},\"alternateName\":\"Sygnature\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"fr-CA\"},{\"@type\":\"Organization\",\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/#organization\",\"name\":\"Sygnature\",\"url\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"fr-CA\",\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/#\\\/schema\\\/logo\\\/image\\\/\",\"url\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/wp-content\\\/uploads\\\/2025\\\/11\\\/Logo.svg\",\"contentUrl\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/wp-content\\\/uploads\\\/2025\\\/11\\\/Logo.svg\",\"width\":1,\"height\":1,\"caption\":\"Sygnature\"},\"image\":{\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/#\\\/schema\\\/logo\\\/image\\\/\"}}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Discovery of Novel Ligase Ligands Using Affinity Selection Mass Spectrometry - Sygnature","description":"In this poster, we demonstrate ASMS screening for novel VHL ligands in TPD programs. Download for more insights.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/discovery-of-novel-ligase-ligands-using-affinity-selection-mass-spectrometry\/","og_locale":"fr_CA","og_type":"article","og_title":"Discovery of Novel Ligase Ligands Using Affinity Selection Mass Spectrometry - Sygnature","og_description":"In this poster, we demonstrate ASMS screening for novel VHL ligands in TPD programs. Download for more insights.","og_url":"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/discovery-of-novel-ligase-ligands-using-affinity-selection-mass-spectrometry\/","og_site_name":"Sygnature","article_modified_time":"2026-01-22T15:18:55+00:00","og_image":[{"width":628,"height":807,"url":"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/11\/Discovery-of-novel-ligase-ligands-using-asms.png","type":"image\/png"}],"twitter_card":"summary_large_image","twitter_misc":{"Estimation du temps de lecture":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/discovery-of-novel-ligase-ligands-using-affinity-selection-mass-spectrometry\/","url":"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/discovery-of-novel-ligase-ligands-using-affinity-selection-mass-spectrometry\/","name":"Discovery of Novel Ligase Ligands Using Affinity Selection Mass Spectrometry - Sygnature","isPartOf":{"@id":"https:\/\/www.sygnaturediscovery.com\/fr\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/discovery-of-novel-ligase-ligands-using-affinity-selection-mass-spectrometry\/#primaryimage"},"image":{"@id":"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/discovery-of-novel-ligase-ligands-using-affinity-selection-mass-spectrometry\/#primaryimage"},"thumbnailUrl":"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/11\/Discovery-of-novel-ligase-ligands-using-asms.png","datePublished":"2024-08-16T12:04:21+00:00","dateModified":"2026-01-22T15:18:55+00:00","description":"In this poster, we demonstrate ASMS screening for novel VHL ligands in TPD programs. Download for more insights.","breadcrumb":{"@id":"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/discovery-of-novel-ligase-ligands-using-affinity-selection-mass-spectrometry\/#breadcrumb"},"inLanguage":"fr-CA","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.sygnaturediscovery.com\/fr\/poster\/discovery-of-novel-ligase-ligands-using-affinity-selection-mass-spectrometry\/"]}]},{"@type":"ImageObject","inLanguage":"fr-CA","@id":"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/discovery-of-novel-ligase-ligands-using-affinity-selection-mass-spectrometry\/#primaryimage","url":"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/11\/Discovery-of-novel-ligase-ligands-using-asms.png","contentUrl":"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/11\/Discovery-of-novel-ligase-ligands-using-asms.png","width":628,"height":807},{"@type":"BreadcrumbList","@id":"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/discovery-of-novel-ligase-ligands-using-affinity-selection-mass-spectrometry\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/www.sygnaturediscovery.com\/fr\/page-daccueil\/"},{"@type":"ListItem","position":2,"name":"Discovery of Novel Ligase Ligands Using Affinity Selection Mass Spectrometry"}]},{"@type":"WebSite","@id":"https:\/\/www.sygnaturediscovery.com\/fr\/#website","url":"https:\/\/www.sygnaturediscovery.com\/fr\/","name":"Sygnature Discovery","description":"","publisher":{"@id":"https:\/\/www.sygnaturediscovery.com\/fr\/#organization"},"alternateName":"Sygnature","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.sygnaturediscovery.com\/fr\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"fr-CA"},{"@type":"Organization","@id":"https:\/\/www.sygnaturediscovery.com\/fr\/#organization","name":"Sygnature","url":"https:\/\/www.sygnaturediscovery.com\/fr\/","logo":{"@type":"ImageObject","inLanguage":"fr-CA","@id":"https:\/\/www.sygnaturediscovery.com\/fr\/#\/schema\/logo\/image\/","url":"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/11\/Logo.svg","contentUrl":"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/11\/Logo.svg","width":1,"height":1,"caption":"Sygnature"},"image":{"@id":"https:\/\/www.sygnaturediscovery.com\/fr\/#\/schema\/logo\/image\/"}}]}},"_links":{"self":[{"href":"https:\/\/www.sygnaturediscovery.com\/fr\/wp-json\/wp\/v2\/poster\/4086","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.sygnaturediscovery.com\/fr\/wp-json\/wp\/v2\/poster"}],"about":[{"href":"https:\/\/www.sygnaturediscovery.com\/fr\/wp-json\/wp\/v2\/types\/poster"}],"version-history":[{"count":0,"href":"https:\/\/www.sygnaturediscovery.com\/fr\/wp-json\/wp\/v2\/poster\/4086\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.sygnaturediscovery.com\/fr\/wp-json\/wp\/v2\/media\/2793"}],"wp:attachment":[{"href":"https:\/\/www.sygnaturediscovery.com\/fr\/wp-json\/wp\/v2\/media?parent=4086"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.sygnaturediscovery.com\/fr\/wp-json\/wp\/v2\/category?post=4086"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}