{"id":4059,"date":"2024-08-07T11:05:25","date_gmt":"2024-08-07T11:05:25","guid":{"rendered":"https:\/\/www.sygnaturediscovery.com\/poster\/a-screening-platform-for-immunomodulatory-compounds-targeting-b-lymphocytes\/"},"modified":"2026-01-22T15:26:33","modified_gmt":"2026-01-22T15:26:33","slug":"a-screening-platform-for-immunomodulatory-compounds-targeting-b-lymphocytes","status":"publish","type":"poster","link":"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/a-screening-platform-for-immunomodulatory-compounds-targeting-b-lymphocytes\/","title":{"rendered":"A Screening Platform for Immunomodulatory Compounds Targeting B Lymphocytes"},"content":{"rendered":"<p><span data-contrast=\"auto\">B cells play a critical role in humoral immunity as part of the adaptive immune system with roles in antigen presentation, cytokine secretion and antibody production. B cells can be activated via different pathways, including T cell-independent (TI) and T cell-dependent (TD) mechanisms. <\/span><\/p>\n<p><span data-contrast=\"auto\">Furthermore, B cell activity can be modulated by a B-cell-activating factor (BAFF), a survival and maturation factor for B cells.<\/span> <span data-contrast=\"auto\">Abnormal B cell activity can lead to many B cell-specific malignancies and autoimmune diseases, including systemic lupus erythematosus and B-cell lymphomas. Here, we establish an <\/span><i><span data-contrast=\"auto\">in vitro<\/span><\/i><span data-contrast=\"auto\"> screening platform to study B cell activity and assess compounds specifically targeting B cell signalling and function.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">\u00a0<\/span><\/p>\n<p><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">\u00a0<\/span><\/p>\n<p><span data-contrast=\"auto\">Following stimulation with either CpG-ODN + IL-15 (a model for TI activation) or CD40L + IL-4, (a model for TD activation) there was a marked increase in B cell activation marker expression (CD71, CD80, CD86) and proliferation (analysed by Flow Cytometry) compared to unstimulated B cells. Moreover, BAFF augmented this response with similar activity after 6 or 9 days of culture. As measured by Flow Cytometry, the frequency of B cell subpopulations (IgD+ memory, IgD- memory cells and plasmablasts) increased with TI and TD stimulation. Moreover, the proportion of these subpopulations was further enhanced with BAFF treatment. B cell-specific inhibitors ((BTK (ibrutinib) and Syk (BAY-61-3606)) and the immunosuppressant dexamethasone reduced B cell activation, proliferation and IgG secretion elicited by CpG-ODN.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">\u00a0<\/span><\/p>\n<p><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">\u00a0<\/span><\/p>\n<p><span data-contrast=\"auto\">We have validated an <\/span><i><span data-contrast=\"auto\">in vitro<\/span><\/i><span data-contrast=\"auto\"> model of B cell activity (proliferation, activation, IgG production) using both TD and TI stimuli and further confirmed this model using specific B cell inhibitors and a general immunosuppressant. This allows for the <a href=\"https:\/\/www.sygnaturediscovery.com\/news-and-events\/blog\/lymphoma-treatment-enters-clinical-phase\/\">screening<\/a> of therapeutics targeting B cell signalling and function for treating autoimmune conditions.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">\u00a0<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>In this poster, we present an in vitro platform to study B cell activity, including proliferation and activation, for therapeutic screening. Download for more insights into targeting B cell signalling and function for autoimmune conditions.<\/p>\n","protected":false},"featured_media":2756,"template":"","category":[681,752,712,688],"class_list":["post-4059","poster","type-poster","status-publish","has-post-thumbnail","hentry","category-bioscience","category-ii","category-primary-cells-and-tissues","category-therapeutic-areas"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.2 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>A Screening Platform for Immunomodulatory Compounds Targeting B Lymphocytes - Sygnature<\/title>\n<meta name=\"description\" content=\"In this poster, we present an in vitro platform to study B cell activity for therapeutic screening. 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