{"id":4006,"date":"2023-10-13T12:27:21","date_gmt":"2023-10-13T12:27:21","guid":{"rendered":"https:\/\/www.sygnaturediscovery.com\/poster\/development-and-validation-of-3hcimbi-36-binding-in-mouse-frontal-cortex-including-affinity-constant-determination-for-classical-psychedelics-comparison-to-3hmdl100907-binding\/"},"modified":"2026-01-22T17:00:32","modified_gmt":"2026-01-22T17:00:32","slug":"development-and-validation-of-3hcimbi-36-binding-in-mouse-frontal-cortex-including-affinity-constant-determination-for-classical-psychedelics-comparison-to-3hmdl100907-binding","status":"publish","type":"poster","link":"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/development-and-validation-of-3hcimbi-36-binding-in-mouse-frontal-cortex-including-affinity-constant-determination-for-classical-psychedelics-comparison-to-3hmdl100907-binding\/","title":{"rendered":"Development and Validation of [3H]Cimbi-36 Binding in Mouse Frontal Cortex Including Affinity Constant Determination for Classical Psychedelics: Comparison to [3H]MDL100,907 Binding"},"content":{"rendered":"

Radioligand choice in competitive binding assays can affect the affinity of a ligand for the receptor.<\/p>\n

At Sygnature Discovery, we have validated a receptor binding screen for 5-HT2A<\/sub> receptors utilising agonist and antagonist radioligands, to investigate receptor pharmacology and translate cell binding data to pre-clinical<\/a> behaviour, DMPK<\/a> and ex vivo receptor binding.<\/p>\n

Antagonists bind to receptors in the active and inactive state whereas agonists only bind to the active state. Cimbi-36 is a potent and selective 5-HT2A<\/sub> agonist, commonly used in PET imaging studies. Sygnature Discovery has developed a radioligand binding assay using [3<\/sup>H]Cimbi-36 to label 5-HT2A<\/sub> receptors in the mouse frontal cortex. We have demonstrated that the affinity of some psychedelic<\/a> 5-HT2A<\/sub> agonists differs when using an agonist or antagonist radioligand. Sygnature Discovery can advise on experimental design so that each stage of drug discovery complements the next and ensures in vitro data can be correlated with behavioural models.<\/p>\n","protected":false},"excerpt":{"rendered":"

At Sygnature Discovery, we’re advancing receptor binding assays by utilizing diverse radioligands. Our 5-HT2A receptor screen, employing both agonist and antagonist radioligands, offers insights into receptor pharmacology. Using [3H]Cimbi-36 as an agonist radioligand, we’ve shed light on the differing affinities of psychedelic 5-HT2A agonists. Trust us to optimize your experimental design and enhance the correlation between in vitro data and behavioral models in the drug discovery process<\/p>\n","protected":false},"featured_media":2676,"template":"","category":[735,740,685,753,688],"class_list":["post-4006","poster","type-poster","status-publish","has-post-thumbnail","hentry","category-cns-pain-models","category-ex-vivo-tissue-analysis","category-in-vivo-pharmacology","category-neuroscience","category-therapeutic-areas"],"acf":[],"yoast_head":"\nDevelopment and Validation of [3H]Cimbi-36 Binding<\/title>\n<meta name=\"description\" content=\"Optimize binding assays with our diverse radioligand screens for receptor pharmacology. Discover more by downloading the digital poster.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/development-and-validation-of-3hcimbi-36-binding-in-mouse-frontal-cortex-including-affinity-constant-determination-for-classical-psychedelics-comparison-to-3hmdl100907-binding\/\" \/>\n<meta property=\"og:locale\" content=\"fr_CA\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Development and Validation of [3H]Cimbi-36 Binding\" \/>\n<meta property=\"og:description\" content=\"Optimize binding assays with our diverse radioligand screens for receptor pharmacology. 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