{"id":3960,"date":"2019-10-30T09:00:39","date_gmt":"2019-10-30T09:00:39","guid":{"rendered":"https:\/\/www.sygnaturediscovery.com\/poster\/microscale-thermophoresis-mst-as-a-novel-alternative-biophysical-method-for-fragment-screening-and-hit-profiling\/"},"modified":"2025-12-12T11:36:44","modified_gmt":"2025-12-12T11:36:44","slug":"microscale-thermophoresis-mst-as-a-novel-alternative-biophysical-method-for-fragment-screening-and-hit-profiling","status":"publish","type":"poster","link":"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/microscale-thermophoresis-mst-as-a-novel-alternative-biophysical-method-for-fragment-screening-and-hit-profiling\/","title":{"rendered":"Microscale Thermophoresis (MST) as a novel, alternative biophysical method for fragment screening and hit profiling"},"content":{"rendered":"

Fragment-based drug discovery (FBDD) relies on sensitive high-throughput screening technologies to quickly and reliably generate hits against a target. MST is a recently established biophysical method for measuring the binding of small molecules to a macromolecule. It is sensitive, solution-based, low-consumption and relatively high-throughput making it ideal for the screening of low molecular weight fragments against a target of interest.
\nHere, we demonstrate an in-house fragment screening project using MST against the immuno-oncology target indoleamine 2,3-dioxygenase 1 (IDO1). We provide follow-up data gathered from a complementary, orthogonal biochemical assay and near-neighbour SAR-by-library work which validates the fragment hits and the MST screening approach as a whole.<\/p>\n","protected":false},"excerpt":{"rendered":"

Discover fragment hits with Microscale Thermophoresis (MST), a breakthrough method for reliable fragment screening and hit profiling.<\/p>\n","protected":false},"featured_media":2607,"template":"","category":[1],"class_list":["post-3960","poster","type-poster","status-publish","has-post-thumbnail","hentry","category-uncategorized"],"acf":[],"yoast_head":"\nMST: A Novel Method for Fragment Screening and Hit Profiling<\/title>\n<meta name=\"description\" content=\"Discover fragment hits with Microscale Thermophoresis (MST), a breakthrough method for reliable fragment screening and hit profiling.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/microscale-thermophoresis-mst-as-a-novel-alternative-biophysical-method-for-fragment-screening-and-hit-profiling\/\" \/>\n<meta property=\"og:locale\" content=\"fr_CA\" \/>\n<meta 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