{"id":18179,"date":"2026-08-10T09:32:29","date_gmt":"2026-08-10T09:32:29","guid":{"rendered":"https:\/\/www.sygnaturediscovery.com\/?post_type=poster&#038;p=18179"},"modified":"2026-08-10T13:57:29","modified_gmt":"2026-08-10T13:57:29","slug":"cellular-target-engagement-assay-development","status":"publish","type":"poster","link":"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/cellular-target-engagement-assay-development\/","title":{"rendered":"Cellular Target Engagement Assay Development"},"content":{"rendered":"\n<h2 class=\"wp-block-heading has-text-2-xl-font-size\">Cellular Target Engagement Assay Development: A RIPK1 Case Study <\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">Demonstrating cellular target engagement is essential for confirming that compounds bind and engage their target in a physiologically relevant environment. In this poster, Sygnature Discovery presents the development and validation of a cellular target engagement assay for receptor-interacting serine\/threonine-protein kinase 1 (RIPK1), a key regulator of inflammatory and programmed cell death pathways<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Using enzyme fragment complementation (EFC)-based technology, the assay quantifies compound-induced thermal stabilization of RIPK1 in living cells, enabling the generation of cellular target engagement EC\u2085\u2080 values with minimal hands-on time. The study demonstrates successful assay optimization and validation using established RIPK1 inhibitors, with cellular target engagement data aligning with functional activity in a necroptosis model.<\/p>\n\n\n\n<h3 class=\"wp-block-heading has-text-lg-font-size\" style=\"padding-top:var(--wp--preset--spacing--30)\">Study Highlights <\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Development of a robust cellular target engagement assay for RIPK1 <\/li>\n\n\n\n<li>Optimization of assay conditions for sensitive detection in live cells <\/li>\n\n\n\n<li>Quantitative measurement of cellular target engagement and EC<sub>50<\/sub> values in live cells <\/li>\n\n\n\n<li>Validation using established RIPK1 inhibitors with expected rank-order potency<\/li>\n\n\n\n<li>Correlation of target engagement data with activity in a functional necroptosis model<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading has-text-lg-font-size\" style=\"padding-top:var(--wp--preset--spacing--30)\">Supporting Cellular Target Engagement Studies <\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">Cellular target engagement studies provide critical evidence that compounds reach and interact with their intended target in a biologically relevant setting. This RIPK1 case study highlights Sygnature Discovery&rsquo;s expertise in developing and validating fit-for-purpose cellular assays that support hit validation, potency ranking, lead optimization, and mechanism-of-action studies.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">By combining assay development, cellular pharmacology, and translational biology expertise, our scientists generate actionable data that helps clients make informed decisions and progress promising drug candidates with greater confidence.<\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"featured_media":18182,"template":"","category":[],"class_list":["post-18179","poster","type-poster","status-publish","has-post-thumbnail","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.0 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Cellular Target Engagement Assay Development - Sygnature<\/title>\n<meta name=\"description\" content=\"Discover how Sygnature Discovery developed and validated a cellular target engagement assay, supporting drug discovery.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/cellular-target-engagement-assay-development\/\" \/>\n<meta property=\"og:locale\" content=\"fr_CA\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Cellular Target Engagement Assay Development - 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