Cereblon (CRBN) is the most widely used E3 ligase in targeted protein degradation and a cornerstone of molecular glue and PROTAC discovery. Yet, unlocking new chemical matter beyond classical IMiDs requires robust structural and biophysical platforms.<\/p>\n\n\n\n
In this study, we established a recombinant CRBNmidi<\/sup> platform combining high-quality protein production, SPR fragment screening (>2100 compounds), and X-ray crystallography validation. This integrated workflow enables the identification and structural confirmation of novel binders to the thalidomide-binding domain (TBD), including non-imide scaffolds.<\/p>\n\n\n\n The results? A scalable, structure-enabled discovery engine that delivers validated fragment hits, supports structure-guided optimization, and expands the accessible chemical space for next-generation degraders.<\/p>\n\n\n\n \ud83d\udc49 Download the free poster to explore how this platform accelerates molecular glue and PROTAC discovery.<\/p>\n\n\n\n <\/p>\n","protected":false},"excerpt":{"rendered":" SPR Fragment Screening & Crystallography Hit Validation with CRBNmidi<\/p>\n","protected":false},"featured_media":16687,"template":"","category":[711,681,770,755,686,680,703,743],"class_list":["post-16665","poster","type-poster","status-publish","has-post-thumbnail","hentry","category-biophysical-assays","category-bioscience","category-crystallography","category-fbdd","category-modalities","category-protein-and-structure","category-protein-characterisation","category-tpd"],"acf":[],"yoast_head":"\n