{"id":12885,"date":"2024-12-01T12:06:00","date_gmt":"2024-12-01T12:06:00","guid":{"rendered":"https:\/\/www.sygnaturediscovery.com\/?post_type=poster&#038;p=12885"},"modified":"2026-01-22T14:25:22","modified_gmt":"2026-01-22T14:25:22","slug":"trpml1-screening-with-ssm-electrophysiology","status":"publish","type":"poster","link":"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/trpml1-screening-with-ssm-electrophysiology\/","title":{"rendered":"TRPML1 Screening with SSM Electrophysiology"},"content":{"rendered":"\n<h2 class=\"wp-block-heading has-dark-blue-500-color has-text-color has-link-color has-text-3-xl-font-size wp-elements-0adc923670599e701185477ce8b94cda\">TRPML1 screening using solid supported membrane electrophysiology (SSME) provides a robust, high-throughput platform for profiling channel modulators. Sygnature Discovery applies this approach to accelerate early-stage discovery of TRPML1-tagreting therapeutic.<\/h2>\n\n\n\n<h2 class=\"wp-block-heading has-dark-blue-500-color has-text-color has-link-color has-text-2-xl-font-size wp-elements-e4eff60c5e433ccf3f3a356443481705\">Abstract<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">Neurodegenerative disorders such as Alzheimer\u2019s and Parkinson\u2019s disease remain conditions with major unmet clinical needs. Abnormalities in the endosomal-autophagic-lysosomal system, progressive neurological dysfunction and regional neuronal loss constitute their common characteristics. Transient receptor potential mucolipin 1 (TRPML1) is a well-known, non-selective cation channel of the endolysosomal system that can transport Ca<sub>2+<\/sub>, K<sub>+<\/sub>, Na<sub>+<\/sub>, Fe<sub>2+<\/sub> and Zn<sub>2+<\/sub>. There is a strong connection between endolysosomal TRPML1 dysfunction and neurodegenerative disorders, thought to be a result of its importance in controlling calcium signalling and homeostasis of lysosomes, autophagy, and modulation of oxidative stress.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Therefore, modulation of TRPML1 represents a promising strategy to improve the function of neurons impacted by neurodegenerative disorders by increasing autophagy and promoting the clearance of protein aggregates and reactive oxygen species (ROS) build-up. Numerous platforms offer insights into TRPML1 function and pharmacology, however, lack of specific tools allowing for investigation of TRPML1\u2019s role in pathological processes is still an obstacle for drug discovery. Solid Supported Membrane Electrophysiology (SSME) present a novel high throughput method to resolve this challenge. Using enriched lysosomal fractions from recombinant HEK cell lines expressing TRMPL1 we have successfully developed SSME assays, using both SURFE\u00b2R N1 (single sensor) and SURFE\u00b2R 96SE (high throughput) platforms to investigate TRPML1\u2019s cation selectivity and pharmacology. Both platforms show excellent reproducibility and platform-to-platform correlation.<\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"featured_media":2814,"template":"","category":[681,709,707,746,753,687,688,777],"class_list":["post-12885","poster","type-poster","status-publish","has-post-thumbnail","hentry","category-bioscience","category-cell-based-assays","category-electrophysiology","category-ion-channels","category-neuroscience","category-target-classes","category-therapeutic-areas","category-trp-channels"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>TRPML1 Screening with SSM Electrophysiology - Sygnature<\/title>\n<meta name=\"description\" content=\"Explore TRPML1 screening with SSM electrophysiology and its potential to address neurodegenerative disorders effectively.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/trpml1-screening-with-ssm-electrophysiology\/\" \/>\n<meta property=\"og:locale\" content=\"fr_CA\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"TRPML1 Screening with SSM Electrophysiology - Sygnature\" \/>\n<meta property=\"og:description\" content=\"Explore TRPML1 screening with SSM electrophysiology and its potential to address neurodegenerative disorders effectively.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/trpml1-screening-with-ssm-electrophysiology\/\" \/>\n<meta property=\"og:site_name\" content=\"Sygnature\" \/>\n<meta property=\"article:modified_time\" content=\"2026-01-22T14:25:22+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/11\/Utilizing-Solid-Supported-Membrane-Electrophysiology-SSME-to-Accelerate-Discovery-of-TRPML1-Modulators.png\" \/>\n\t<meta property=\"og:image:width\" content=\"888\" \/>\n\t<meta property=\"og:image:height\" content=\"628\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Estimation du temps de lecture\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/poster\\\/trpml1-screening-with-ssm-electrophysiology\\\/\",\"url\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/poster\\\/trpml1-screening-with-ssm-electrophysiology\\\/\",\"name\":\"TRPML1 Screening with SSM Electrophysiology - Sygnature\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/poster\\\/trpml1-screening-with-ssm-electrophysiology\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/poster\\\/trpml1-screening-with-ssm-electrophysiology\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/wp-content\\\/uploads\\\/2025\\\/11\\\/Utilizing-Solid-Supported-Membrane-Electrophysiology-SSME-to-Accelerate-Discovery-of-TRPML1-Modulators.png\",\"datePublished\":\"2024-12-01T12:06:00+00:00\",\"dateModified\":\"2026-01-22T14:25:22+00:00\",\"description\":\"Explore TRPML1 screening with SSM electrophysiology and its potential to address neurodegenerative disorders effectively.\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/poster\\\/trpml1-screening-with-ssm-electrophysiology\\\/#breadcrumb\"},\"inLanguage\":\"fr-CA\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/poster\\\/trpml1-screening-with-ssm-electrophysiology\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"fr-CA\",\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/poster\\\/trpml1-screening-with-ssm-electrophysiology\\\/#primaryimage\",\"url\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/wp-content\\\/uploads\\\/2025\\\/11\\\/Utilizing-Solid-Supported-Membrane-Electrophysiology-SSME-to-Accelerate-Discovery-of-TRPML1-Modulators.png\",\"contentUrl\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/wp-content\\\/uploads\\\/2025\\\/11\\\/Utilizing-Solid-Supported-Membrane-Electrophysiology-SSME-to-Accelerate-Discovery-of-TRPML1-Modulators.png\",\"width\":888,\"height\":628,\"caption\":\"Utilizing-Solid-Supported-Membrane-Electrophysiology-SSME-to-Accelerate-Discovery-of-TRPML1-Modulators\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/poster\\\/trpml1-screening-with-ssm-electrophysiology\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/page-daccueil\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"TRPML1 Screening with SSM Electrophysiology\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/#website\",\"url\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/\",\"name\":\"Sygnature Discovery\",\"description\":\"\",\"publisher\":{\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/#organization\"},\"alternateName\":\"Sygnature\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"fr-CA\"},{\"@type\":\"Organization\",\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/#organization\",\"name\":\"Sygnature\",\"url\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"fr-CA\",\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/#\\\/schema\\\/logo\\\/image\\\/\",\"url\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/wp-content\\\/uploads\\\/2025\\\/11\\\/Logo.svg\",\"contentUrl\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/wp-content\\\/uploads\\\/2025\\\/11\\\/Logo.svg\",\"width\":1,\"height\":1,\"caption\":\"Sygnature\"},\"image\":{\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/#\\\/schema\\\/logo\\\/image\\\/\"}}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"TRPML1 Screening with SSM Electrophysiology - Sygnature","description":"Explore TRPML1 screening with SSM electrophysiology and its potential to address neurodegenerative disorders effectively.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/trpml1-screening-with-ssm-electrophysiology\/","og_locale":"fr_CA","og_type":"article","og_title":"TRPML1 Screening with SSM Electrophysiology - Sygnature","og_description":"Explore TRPML1 screening with SSM electrophysiology and its potential to address neurodegenerative disorders effectively.","og_url":"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/trpml1-screening-with-ssm-electrophysiology\/","og_site_name":"Sygnature","article_modified_time":"2026-01-22T14:25:22+00:00","og_image":[{"width":888,"height":628,"url":"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/11\/Utilizing-Solid-Supported-Membrane-Electrophysiology-SSME-to-Accelerate-Discovery-of-TRPML1-Modulators.png","type":"image\/png"}],"twitter_card":"summary_large_image","twitter_misc":{"Estimation du temps de lecture":"2 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/trpml1-screening-with-ssm-electrophysiology\/","url":"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/trpml1-screening-with-ssm-electrophysiology\/","name":"TRPML1 Screening with SSM Electrophysiology - Sygnature","isPartOf":{"@id":"https:\/\/www.sygnaturediscovery.com\/fr\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/trpml1-screening-with-ssm-electrophysiology\/#primaryimage"},"image":{"@id":"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/trpml1-screening-with-ssm-electrophysiology\/#primaryimage"},"thumbnailUrl":"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/11\/Utilizing-Solid-Supported-Membrane-Electrophysiology-SSME-to-Accelerate-Discovery-of-TRPML1-Modulators.png","datePublished":"2024-12-01T12:06:00+00:00","dateModified":"2026-01-22T14:25:22+00:00","description":"Explore TRPML1 screening with SSM electrophysiology and its potential to address neurodegenerative disorders effectively.","breadcrumb":{"@id":"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/trpml1-screening-with-ssm-electrophysiology\/#breadcrumb"},"inLanguage":"fr-CA","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.sygnaturediscovery.com\/fr\/poster\/trpml1-screening-with-ssm-electrophysiology\/"]}]},{"@type":"ImageObject","inLanguage":"fr-CA","@id":"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/trpml1-screening-with-ssm-electrophysiology\/#primaryimage","url":"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/11\/Utilizing-Solid-Supported-Membrane-Electrophysiology-SSME-to-Accelerate-Discovery-of-TRPML1-Modulators.png","contentUrl":"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/11\/Utilizing-Solid-Supported-Membrane-Electrophysiology-SSME-to-Accelerate-Discovery-of-TRPML1-Modulators.png","width":888,"height":628,"caption":"Utilizing-Solid-Supported-Membrane-Electrophysiology-SSME-to-Accelerate-Discovery-of-TRPML1-Modulators"},{"@type":"BreadcrumbList","@id":"https:\/\/www.sygnaturediscovery.com\/fr\/poster\/trpml1-screening-with-ssm-electrophysiology\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/www.sygnaturediscovery.com\/fr\/page-daccueil\/"},{"@type":"ListItem","position":2,"name":"TRPML1 Screening with SSM Electrophysiology"}]},{"@type":"WebSite","@id":"https:\/\/www.sygnaturediscovery.com\/fr\/#website","url":"https:\/\/www.sygnaturediscovery.com\/fr\/","name":"Sygnature Discovery","description":"","publisher":{"@id":"https:\/\/www.sygnaturediscovery.com\/fr\/#organization"},"alternateName":"Sygnature","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.sygnaturediscovery.com\/fr\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"fr-CA"},{"@type":"Organization","@id":"https:\/\/www.sygnaturediscovery.com\/fr\/#organization","name":"Sygnature","url":"https:\/\/www.sygnaturediscovery.com\/fr\/","logo":{"@type":"ImageObject","inLanguage":"fr-CA","@id":"https:\/\/www.sygnaturediscovery.com\/fr\/#\/schema\/logo\/image\/","url":"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/11\/Logo.svg","contentUrl":"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/11\/Logo.svg","width":1,"height":1,"caption":"Sygnature"},"image":{"@id":"https:\/\/www.sygnaturediscovery.com\/fr\/#\/schema\/logo\/image\/"}}]}},"_links":{"self":[{"href":"https:\/\/www.sygnaturediscovery.com\/fr\/wp-json\/wp\/v2\/poster\/12885","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.sygnaturediscovery.com\/fr\/wp-json\/wp\/v2\/poster"}],"about":[{"href":"https:\/\/www.sygnaturediscovery.com\/fr\/wp-json\/wp\/v2\/types\/poster"}],"version-history":[{"count":0,"href":"https:\/\/www.sygnaturediscovery.com\/fr\/wp-json\/wp\/v2\/poster\/12885\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.sygnaturediscovery.com\/fr\/wp-json\/wp\/v2\/media\/2814"}],"wp:attachment":[{"href":"https:\/\/www.sygnaturediscovery.com\/fr\/wp-json\/wp\/v2\/media?parent=12885"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.sygnaturediscovery.com\/fr\/wp-json\/wp\/v2\/category?post=12885"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}