{"id":2411,"date":"2018-03-27T13:33:19","date_gmt":"2018-03-27T13:33:19","guid":{"rendered":"https:\/\/www.sygnaturediscovery.com\/journal-paper\/combinatorial-biophysical-approach-ftsa-spr-identifying-small-molecule-ligands-pains\/"},"modified":"2018-03-27T13:33:19","modified_gmt":"2018-03-27T13:33:19","slug":"combinatorial-biophysical-approach-ftsa-spr-identifying-small-molecule-ligands-pains","status":"publish","type":"journal-paper","link":"https:\/\/www.sygnaturediscovery.com\/fr\/journal-paper\/combinatorial-biophysical-approach-ftsa-spr-identifying-small-molecule-ligands-pains\/","title":{"rendered":"Combinatorial Biophysical Approach: FTSA and SPR for Small Molecule Ligand and PAIN Identification"},"content":{"rendered":"<p>Redhead,M.,\u00a0Satchell, R.,\u00a0Mork\u016bnait\u0117, V.,\u00a0Swift, D.,\u00a0Petrauskas, V.,\u00a0Golding, E.,\u00a0Onions, S.,\u00a0Matulis, D.,\u00a0Unitt, J.<\/p>\n<p><strong>Abstract<\/strong><\/p>\n<div class=\"\">\n<p>Biophysical methods have emerged as attractive screening techniques in drug discovery both as primary hit finding methodologies, as in the case of weakly active compounds such as fragments, and as orthogonal methods for hit validation for compounds discovered through conventional biochemical or cellular assays. Here we describe a dual method employing fluorescent thermal shift assay (FTSA), also known as differential scanning fluorimetry (DSF) and surface plasmon resonance (SPR), to interrogate ligands of the kinase p38\u03b1 as well as several known pan-assay interference compounds (PAINs) such as aggregators, redox cyclers, and fluorescence quenchers. This combinatorial approach allows for independent verification of several biophysical parameters such as KD, kon, koff, \u0394G, \u0394S, and \u0394H, which may further guide chemical development of a ligand series. Affinity values obtained from <a href=\"https:\/\/www.sygnaturediscovery.com\/drug-discovery\/bioscience\/biophysical-assays\/fluorescent-thermal-shift-assays-ftsa\/\">FTSA<\/a> curves allow for insight into compound binding compared with reporting shifts in melting temperature. Ligand-p38 interaction data were in good agreement with previous literature. Aggregators and fluorescence quenchers appeared to reduce fluorescence signal in the FTSAs, causing artificially high shifts in Tm values, whereas redox compounds caused either shifts in affinity that did not agree between <a href=\"https:\/\/www.sygnaturediscovery.com\/drug-discovery\/bioscience\/biophysical-assays\/fluorescent-thermal-shift-assays-ftsa\/\">FTSA<\/a> and SPR or a depression of FTSA signal.<\/p>\n<p><strong>Ref:<\/strong> Anal Biochem.\u00a02015 Jun 15;479:63-73<\/p>\n<\/div>\n<p>Would you like a copy? Get in touch!<\/p>\n<p><a href=\"mailto:BusDev@sygnaturediscovery.com\">BusDev@sygnaturediscovery.com<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Biophysical dual method: FTSA and SPR for drug discovery. Explore ligands and PAINs, gain insights on affinity values and compound binding.<\/p>\n","protected":false},"featured_media":0,"template":"","category":[1],"resource_tag":[],"class_list":["post-2411","journal-paper","type-journal-paper","status-publish","hentry","category-uncategorized"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Combinatorial Biophysical Approach for Ligand, PAIN Identification<\/title>\n<meta name=\"description\" content=\"Biophysical dual method: FTSA and SPR for drug discovery. Explore ligands and PAINs, gain insights on affinity values and compound binding.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.sygnaturediscovery.com\/fr\/journal-paper\/combinatorial-biophysical-approach-ftsa-spr-identifying-small-molecule-ligands-pains\/\" \/>\n<meta property=\"og:locale\" content=\"fr_CA\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Combinatorial Biophysical Approach for Ligand, PAIN Identification\" \/>\n<meta property=\"og:description\" content=\"Biophysical dual method: FTSA and SPR for drug discovery. Explore ligands and PAINs, gain insights on affinity values and compound binding.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.sygnaturediscovery.com\/fr\/journal-paper\/combinatorial-biophysical-approach-ftsa-spr-identifying-small-molecule-ligands-pains\/\" \/>\n<meta property=\"og:site_name\" content=\"Sygnature\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2026\/02\/sygnature-hit-to-lead-team-collaboration-scaled.webp\" \/>\n\t<meta property=\"og:image:width\" content=\"2560\" \/>\n\t<meta property=\"og:image:height\" content=\"1707\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/webp\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Estimation du temps de lecture\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/journal-paper\\\/combinatorial-biophysical-approach-ftsa-spr-identifying-small-molecule-ligands-pains\\\/\",\"url\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/journal-paper\\\/combinatorial-biophysical-approach-ftsa-spr-identifying-small-molecule-ligands-pains\\\/\",\"name\":\"Combinatorial Biophysical Approach for Ligand, PAIN Identification\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/#website\"},\"datePublished\":\"2018-03-27T13:33:19+00:00\",\"description\":\"Biophysical dual method: FTSA and SPR for drug discovery. Explore ligands and PAINs, gain insights on affinity values and compound binding.\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/journal-paper\\\/combinatorial-biophysical-approach-ftsa-spr-identifying-small-molecule-ligands-pains\\\/#breadcrumb\"},\"inLanguage\":\"fr-CA\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/journal-paper\\\/combinatorial-biophysical-approach-ftsa-spr-identifying-small-molecule-ligands-pains\\\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/journal-paper\\\/combinatorial-biophysical-approach-ftsa-spr-identifying-small-molecule-ligands-pains\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/page-daccueil\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Combinatorial Biophysical Approach: FTSA and SPR for Small Molecule Ligand and PAIN Identification\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/#website\",\"url\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/\",\"name\":\"Sygnature Discovery\",\"description\":\"\",\"publisher\":{\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/#organization\"},\"alternateName\":\"Sygnature\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"fr-CA\"},{\"@type\":\"Organization\",\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/#organization\",\"name\":\"Sygnature Discovery\",\"url\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"fr-CA\",\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/#\\\/schema\\\/logo\\\/image\\\/\",\"url\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/wp-content\\\/uploads\\\/2025\\\/11\\\/Logo.svg\",\"contentUrl\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/wp-content\\\/uploads\\\/2025\\\/11\\\/Logo.svg\",\"width\":1,\"height\":1,\"caption\":\"Sygnature Discovery\"},\"image\":{\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/#\\\/schema\\\/logo\\\/image\\\/\"}}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Combinatorial Biophysical Approach for Ligand, PAIN Identification","description":"Biophysical dual method: FTSA and SPR for drug discovery. Explore ligands and PAINs, gain insights on affinity values and compound binding.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.sygnaturediscovery.com\/fr\/journal-paper\/combinatorial-biophysical-approach-ftsa-spr-identifying-small-molecule-ligands-pains\/","og_locale":"fr_CA","og_type":"article","og_title":"Combinatorial Biophysical Approach for Ligand, PAIN Identification","og_description":"Biophysical dual method: FTSA and SPR for drug discovery. Explore ligands and PAINs, gain insights on affinity values and compound binding.","og_url":"https:\/\/www.sygnaturediscovery.com\/fr\/journal-paper\/combinatorial-biophysical-approach-ftsa-spr-identifying-small-molecule-ligands-pains\/","og_site_name":"Sygnature","og_image":[{"width":2560,"height":1707,"url":"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2026\/02\/sygnature-hit-to-lead-team-collaboration-scaled.webp","type":"image\/webp"}],"twitter_card":"summary_large_image","twitter_misc":{"Estimation du temps de lecture":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/www.sygnaturediscovery.com\/fr\/journal-paper\/combinatorial-biophysical-approach-ftsa-spr-identifying-small-molecule-ligands-pains\/","url":"https:\/\/www.sygnaturediscovery.com\/fr\/journal-paper\/combinatorial-biophysical-approach-ftsa-spr-identifying-small-molecule-ligands-pains\/","name":"Combinatorial Biophysical Approach for Ligand, PAIN Identification","isPartOf":{"@id":"https:\/\/www.sygnaturediscovery.com\/fr\/#website"},"datePublished":"2018-03-27T13:33:19+00:00","description":"Biophysical dual method: FTSA and SPR for drug discovery. Explore ligands and PAINs, gain insights on affinity values and compound binding.","breadcrumb":{"@id":"https:\/\/www.sygnaturediscovery.com\/fr\/journal-paper\/combinatorial-biophysical-approach-ftsa-spr-identifying-small-molecule-ligands-pains\/#breadcrumb"},"inLanguage":"fr-CA","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.sygnaturediscovery.com\/fr\/journal-paper\/combinatorial-biophysical-approach-ftsa-spr-identifying-small-molecule-ligands-pains\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/www.sygnaturediscovery.com\/fr\/journal-paper\/combinatorial-biophysical-approach-ftsa-spr-identifying-small-molecule-ligands-pains\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/www.sygnaturediscovery.com\/fr\/page-daccueil\/"},{"@type":"ListItem","position":2,"name":"Combinatorial Biophysical Approach: FTSA and SPR for Small Molecule Ligand and PAIN Identification"}]},{"@type":"WebSite","@id":"https:\/\/www.sygnaturediscovery.com\/fr\/#website","url":"https:\/\/www.sygnaturediscovery.com\/fr\/","name":"Sygnature Discovery","description":"","publisher":{"@id":"https:\/\/www.sygnaturediscovery.com\/fr\/#organization"},"alternateName":"Sygnature","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.sygnaturediscovery.com\/fr\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"fr-CA"},{"@type":"Organization","@id":"https:\/\/www.sygnaturediscovery.com\/fr\/#organization","name":"Sygnature Discovery","url":"https:\/\/www.sygnaturediscovery.com\/fr\/","logo":{"@type":"ImageObject","inLanguage":"fr-CA","@id":"https:\/\/www.sygnaturediscovery.com\/fr\/#\/schema\/logo\/image\/","url":"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/11\/Logo.svg","contentUrl":"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/11\/Logo.svg","width":1,"height":1,"caption":"Sygnature Discovery"},"image":{"@id":"https:\/\/www.sygnaturediscovery.com\/fr\/#\/schema\/logo\/image\/"}}]}},"_links":{"self":[{"href":"https:\/\/www.sygnaturediscovery.com\/fr\/wp-json\/wp\/v2\/journal-paper\/2411","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.sygnaturediscovery.com\/fr\/wp-json\/wp\/v2\/journal-paper"}],"about":[{"href":"https:\/\/www.sygnaturediscovery.com\/fr\/wp-json\/wp\/v2\/types\/journal-paper"}],"version-history":[{"count":0,"href":"https:\/\/www.sygnaturediscovery.com\/fr\/wp-json\/wp\/v2\/journal-paper\/2411\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.sygnaturediscovery.com\/fr\/wp-json\/wp\/v2\/media?parent=2411"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.sygnaturediscovery.com\/fr\/wp-json\/wp\/v2\/category?post=2411"},{"taxonomy":"resource_tag","embeddable":true,"href":"https:\/\/www.sygnaturediscovery.com\/fr\/wp-json\/wp\/v2\/resource_tag?post=2411"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}