{"id":13439,"date":"2023-06-22T10:11:00","date_gmt":"2023-06-22T10:11:00","guid":{"rendered":"https:\/\/www.sygnaturediscovery.com\/?post_type=journal-paper&#038;p=13439"},"modified":"2026-01-30T10:17:41","modified_gmt":"2026-01-30T10:17:41","slug":"differential-roles-of-ctp-synthetases-ctps1-and-ctps2-in-cell-proliferation","status":"publish","type":"journal-paper","link":"https:\/\/www.sygnaturediscovery.com\/fr\/journal-paper\/differential-roles-of-ctp-synthetases-ctps1-and-ctps2-in-cell-proliferation\/","title":{"rendered":"Differential roles of CTP synthetases CTPS1 and CTPS2 in cell proliferation"},"content":{"rendered":"\n<p>Norbert Minet,\u00a0Anne-Claire Boschat,\u00a0<strong>Rebecca Lane<\/strong>,\u00a0<strong>David Laughton<\/strong>,\u00a0Philip Beer,\u00a0H\u00e9l\u00e8ne Asnagli,\u00a0Claire Soudais,\u00a0Tim Bourne,\u00a0Alain Fischer,\u00a0Emmanuel Martin,\u00a0Sylvain Latour\u00a0<\/p>\n\n\n\n<p><strong>Abstract<\/strong><\/p>\n\n\n\n<p>The CTP nucleotide is a key precursor of nucleic acids metabolism essential for DNA replication. De novo CTP production relies on CTP synthetases 1 and 2 (CTPS1 and CTPS2) that catalyze the conversion of UTP into CTP. CTP synthetase activity is high in proliferating cells including cancer cells; however, the respective roles of CTPS1 and CTPS2 in cell proliferation are not known. By inactivation of&nbsp;<em>CTPS1<\/em>&nbsp;and\/or&nbsp;<em>CTPS2<\/em>&nbsp;and complementation experiments, we showed that both CTPS1 and CTPS2 are differentially required for cell proliferation. CTPS1 was more efficient in promoting proliferation than CTPS2, in association with a higher intrinsic enzymatic activity that was more resistant to inhibition by 3-deaza-uridine, an UTP analog. The contribution of CTPS2 to cell proliferation was modest when CTPS1 was expressed but essential in absence of CTPS1. Public databases analysis of more than 1,000 inactivated cancer cell lines for CTPS1 or CTPS2 confirmed that cell growth is highly dependent of CTPS1 but less or not of CTPS2. Therefore, our results demonstrate that CTPS1 is the main contributor to cell proliferation.<\/p>\n\n\n\n<p>Ref: <a href=\"https:\/\/www.life-science-alliance.org\/content\/6\/9\/e202302066\">Life Sci Alliance 2023<\/a><\/p>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"featured_media":0,"template":"","category":[681],"resource_tag":[],"class_list":["post-13439","journal-paper","type-journal-paper","status-publish","hentry","category-bioscience"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Differential roles of CTP synthetases CTPS1 and CTPS2 in cell proliferation - Sygnature<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.sygnaturediscovery.com\/fr\/journal-paper\/differential-roles-of-ctp-synthetases-ctps1-and-ctps2-in-cell-proliferation\/\" \/>\n<meta property=\"og:locale\" content=\"fr_CA\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Differential roles of CTP synthetases CTPS1 and CTPS2 in cell proliferation - 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