In a previous eBook, we discussed approaches to successful hit finding. Here, we discuss some considerations in designing screening cascades to progress these hits and develop drug candidates. Whichever hit finding approach or methodology for designing and synthesizing new molecules you choose to develop your drug candidate, you will need a robust and flexible system to test your hypotheses in biological systems and ensure you remain on track. As you start to consider what an appropriate screening cascade should look like, it is important to define what key properties you are looking for in your candidate drug, as these will impact the choice of assays you may deploy in your screening cascade. For example:<\/p>\n\n\n\n