{"id":6703,"date":"2025-12-08T16:32:19","date_gmt":"2025-12-08T16:32:19","guid":{"rendered":"https:\/\/www.sygnaturediscovery.com\/?post_type=case-study&#038;p=6703"},"modified":"2026-02-02T18:05:36","modified_gmt":"2026-02-02T18:05:36","slug":"bk-channel-screening-%ce%b2-subunit-profiling","status":"publish","type":"case-study","link":"https:\/\/www.sygnaturediscovery.com\/fr\/case-study\/bk-channel-screening-%ce%b2-subunit-profiling\/","title":{"rendered":"BK Channel Screening &amp; \u03b2-Subunit Profiling"},"content":{"rendered":"\n<h2 class=\"wp-block-heading has-dark-blue-500-color has-text-color has-link-color has-text-3-xl-font-size wp-elements-d95f9d6ff66af86dd334e257be85e3e4\">BK channel screening allows investigation of the functional and pharmacological impact of \u03b2-subunit expression using electrophysiology. Sygnature Discovery provides detailed profiling to support BK channel-focussed drug discovery and lead optimization.\u00a0<\/h2>\n\n\n\n<h3 class=\"wp-block-heading has-dark-blue-500-color has-text-color has-link-color has-text-2-xl-font-size wp-elements-25e4cdc2f8f9510344f929488ffd6d50\">Introduction<\/h3>\n\n\n\n<p>BK channels, widely recognized as high-conductance voltage and calcium-activated potassium channels, are transmembrane proteins consisting of four pore-forming \u03b1 subunits (Figure 1). Each \u03b1 subunits is comprised of \u00a0seven transmembrane segments, encompassing a \u00a0voltage sensor domain and a pore-forming domain.<\/p>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-7d11ce30 wp-block-columns-is-layout-flex\" style=\"margin-top:var(--wp--preset--spacing--40);margin-bottom:var(--wp--preset--spacing--40)\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure data-wp-context='{\"imageId\":\"69f5dea19e73b\"}' data-wp-interactive=\"core\/image\" data-wp-key=\"69f5dea19e73b\" class=\"wp-block-image size-full has-custom-border wp-lightbox-container\"><div class=\"gif_wrap 696\" data-wpmf-gif-src=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/Schematic-representation-of-the-BK-channel-structure-composed.gif\" data-wpmf-still-src=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/Schematic-representation-of-the-BK-channel-structure-composed-150x150.gif\" data-wpmf-attachment-id=\"6706\"><a href=\"javascript:void(0);\" class=\"gif_link_wrap 696\" title=\"Click to play\" rel=\"nofollow\" data-wpmf-gif-src=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/Schematic-representation-of-the-BK-channel-structure-composed.gif\"><\/a><span class=\"play_gif 696\">GIF<\/span><img src=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/Schematic-representation-of-the-BK-channel-structure-composed-150x150.gif\" class=\"_showing frame no-lazy wp-image-6706\" alt=\"\" data-wpmf-gif-src=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/Schematic-representation-of-the-BK-channel-structure-composed.gif\" data-lazy-src=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/Schematic-representation-of-the-BK-channel-structure-composed.gif\" data-wpmf-still-src=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/Schematic-representation-of-the-BK-channel-structure-composed-150x150.gif\"><\/div><img src=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/Schematic-representation-of-the-BK-channel-structure-composed-150x150.gif\" class=\"_hidden no-lazy\" alt=\"\" style=\"display:none;\" data-wpmf-gif-src=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/Schematic-representation-of-the-BK-channel-structure-composed.gif\" data-lazy-src=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/Schematic-representation-of-the-BK-channel-structure-composed.gif\" data-wpmf-still-src=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/Schematic-representation-of-the-BK-channel-structure-composed-150x150.gif\"><button class=\"lightbox-trigger\" type=\"button\" aria-haspopup=\"dialog\" aria-label=\"Agrandir\" data-wp-init=\"callbacks.initTriggerButton\" data-wp-on--click=\"actions.showLightbox\" data-wp-style--right=\"state.imageButtonRight\" data-wp-style--top=\"state.imageButtonTop\">\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewbox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\"><\/path>\n\t\t\t<\/svg>\n\t\t<\/button><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<p>Far from passive entities, BK channels have a unique dual activation mechanism, responding to both membrane depolarization and intracellular calcium concentrations. This distinctive trait enables BK channels to finely \u00a0modulate cellular excitability and signalling pathways. \u00a0Activation triggers a cascade where BK channels facilitate the efflux of potassium ions, inducing membrane \u00a0hyperpolarization and effectively diminishing cellular \u00a0excitability. \u00a0Beyond their primary duty in overseeing the smooth muscle tone across vital tissues such as blood vessels, urinary blader, and the gastrointestinal tract, BK channels play a pivotal role in modulating neuronal excitability and synaptic transmission. \u00a0Sygnature Discovery have developed a suite of \u00a0comprehensive assays showcasing calcium sensitivity, kinetics, activator and inhibitor profiling, reproducibility benchmarks, and I-V assays. At Sygnature Discovery, we don\u2019t just offer customizable assays; we provide a \u00a0pathway to novel insights, providing your research with data you can trust.<\/p>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-group alignfull is-style-default has-ivory-000-color has-text-color has-background is-layout-flow wp-block-group-is-layout-flow\" style=\"border-radius:1.5em;background:linear-gradient(90deg,rgb(0,60,97) 0%,rgb(4,30,46) 100%);margin-top:var(--wp--preset--spacing--40);margin-bottom:var(--wp--preset--spacing--40);padding-right:var(--wp--preset--spacing--60);padding-left:var(--wp--preset--spacing--60)\" allowchildrentofillwidth=\"\" overflow=\"\" flexwrap=\"\">\n<div style=\"height:64px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p class=\"has-text-align-center has-small-font-size\" style=\"line-height:.9\"><strong>CASE STUDY<\/strong><\/p>\n\n\n\n<h2 class=\"wp-block-heading has-text-align-center\" id=\"schedule-a-visit\" style=\"font-size:clamp(20px, 1.25rem + ((1vw - 3.2px) * 0.938), 32px);line-height:1.15\">Download the PDF: BK Channel Screening &amp; \u03b2-Subunit Profiling<\/h2>\n\n\n\n<div class=\"wp-block-buttons is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-a89b3969 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-50\" modaltype=\"\"><a class=\"wp-block-button__link wp-element-button\" href=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/Pharmacological-effects-of-beta-subunit-expression-on-BK-channel.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Download<\/a><\/div>\n<\/div>\n\n\n\n<div style=\"height:64px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"featured_media":0,"template":"","category":[707,746,780,687],"resource_tag":[],"class_list":["post-6703","case-study","type-case-study","status-publish","hentry","category-electrophysiology","category-ion-channels","category-potassium-channels","category-target-classes"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>BK Channel Screening &amp; \u03b2-Subunit Profiling - Sygnature<\/title>\n<meta name=\"description\" content=\"BK channel screening explores functional and pharmacological effects of beta-subunit expression to support ion channel drug discovery.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.sygnaturediscovery.com\/fr\/case-study\/bk-channel-screening-%ce%b2-subunit-profiling\/\" \/>\n<meta property=\"og:locale\" content=\"fr_CA\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"BK Channel Screening &amp; \u03b2-Subunit Profiling - Sygnature\" \/>\n<meta property=\"og:description\" content=\"BK channel screening explores functional and pharmacological effects of beta-subunit expression to support ion channel drug discovery.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.sygnaturediscovery.com\/fr\/case-study\/bk-channel-screening-%ce%b2-subunit-profiling\/\" \/>\n<meta property=\"og:site_name\" content=\"Sygnature\" \/>\n<meta property=\"article:modified_time\" content=\"2026-02-02T18:05:36+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/Schematic-representation-of-the-BK-channel-structure-composed.gif\" \/>\n\t<meta property=\"og:image:width\" content=\"696\" \/>\n\t<meta property=\"og:image:height\" content=\"478\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/gif\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Estimation du temps de lecture\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/case-study\\\/bk-channel-screening-%ce%b2-subunit-profiling\\\/\",\"url\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/case-study\\\/bk-channel-screening-%ce%b2-subunit-profiling\\\/\",\"name\":\"BK Channel Screening &amp; \u03b2-Subunit Profiling - Sygnature\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/case-study\\\/bk-channel-screening-%ce%b2-subunit-profiling\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/case-study\\\/bk-channel-screening-%ce%b2-subunit-profiling\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/wp-content\\\/uploads\\\/2025\\\/12\\\/Schematic-representation-of-the-BK-channel-structure-composed.gif\",\"datePublished\":\"2025-12-08T16:32:19+00:00\",\"dateModified\":\"2026-02-02T18:05:36+00:00\",\"description\":\"BK channel screening explores functional and pharmacological effects of beta-subunit expression to support ion channel drug discovery.\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/case-study\\\/bk-channel-screening-%ce%b2-subunit-profiling\\\/#breadcrumb\"},\"inLanguage\":\"fr-CA\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/case-study\\\/bk-channel-screening-%ce%b2-subunit-profiling\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"fr-CA\",\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/case-study\\\/bk-channel-screening-%ce%b2-subunit-profiling\\\/#primaryimage\",\"url\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/wp-content\\\/uploads\\\/2025\\\/12\\\/Schematic-representation-of-the-BK-channel-structure-composed.gif\",\"contentUrl\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/wp-content\\\/uploads\\\/2025\\\/12\\\/Schematic-representation-of-the-BK-channel-structure-composed.gif\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/case-study\\\/bk-channel-screening-%ce%b2-subunit-profiling\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/page-daccueil\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"BK Channel Screening &amp; \u03b2-Subunit Profiling\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/#website\",\"url\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/\",\"name\":\"Sygnature Discovery\",\"description\":\"\",\"publisher\":{\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/#organization\"},\"alternateName\":\"Sygnature\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"fr-CA\"},{\"@type\":\"Organization\",\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/#organization\",\"name\":\"Sygnature\",\"url\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"fr-CA\",\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/#\\\/schema\\\/logo\\\/image\\\/\",\"url\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/wp-content\\\/uploads\\\/2025\\\/11\\\/Logo.svg\",\"contentUrl\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/wp-content\\\/uploads\\\/2025\\\/11\\\/Logo.svg\",\"width\":1,\"height\":1,\"caption\":\"Sygnature\"},\"image\":{\"@id\":\"https:\\\/\\\/www.sygnaturediscovery.com\\\/fr\\\/#\\\/schema\\\/logo\\\/image\\\/\"}}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"BK Channel Screening &amp; \u03b2-Subunit Profiling - Sygnature","description":"BK channel screening explores functional and pharmacological effects of beta-subunit expression to support ion channel drug discovery.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.sygnaturediscovery.com\/fr\/case-study\/bk-channel-screening-%ce%b2-subunit-profiling\/","og_locale":"fr_CA","og_type":"article","og_title":"BK Channel Screening &amp; \u03b2-Subunit Profiling - Sygnature","og_description":"BK channel screening explores functional and pharmacological effects of beta-subunit expression to support ion channel drug discovery.","og_url":"https:\/\/www.sygnaturediscovery.com\/fr\/case-study\/bk-channel-screening-%ce%b2-subunit-profiling\/","og_site_name":"Sygnature","article_modified_time":"2026-02-02T18:05:36+00:00","og_image":[{"width":696,"height":478,"url":"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/Schematic-representation-of-the-BK-channel-structure-composed.gif","type":"image\/gif"}],"twitter_card":"summary_large_image","twitter_misc":{"Estimation du temps de lecture":"2 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/www.sygnaturediscovery.com\/fr\/case-study\/bk-channel-screening-%ce%b2-subunit-profiling\/","url":"https:\/\/www.sygnaturediscovery.com\/fr\/case-study\/bk-channel-screening-%ce%b2-subunit-profiling\/","name":"BK Channel Screening &amp; \u03b2-Subunit Profiling - Sygnature","isPartOf":{"@id":"https:\/\/www.sygnaturediscovery.com\/fr\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.sygnaturediscovery.com\/fr\/case-study\/bk-channel-screening-%ce%b2-subunit-profiling\/#primaryimage"},"image":{"@id":"https:\/\/www.sygnaturediscovery.com\/fr\/case-study\/bk-channel-screening-%ce%b2-subunit-profiling\/#primaryimage"},"thumbnailUrl":"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/Schematic-representation-of-the-BK-channel-structure-composed.gif","datePublished":"2025-12-08T16:32:19+00:00","dateModified":"2026-02-02T18:05:36+00:00","description":"BK channel screening explores functional and pharmacological effects of beta-subunit expression to support ion channel drug discovery.","breadcrumb":{"@id":"https:\/\/www.sygnaturediscovery.com\/fr\/case-study\/bk-channel-screening-%ce%b2-subunit-profiling\/#breadcrumb"},"inLanguage":"fr-CA","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.sygnaturediscovery.com\/fr\/case-study\/bk-channel-screening-%ce%b2-subunit-profiling\/"]}]},{"@type":"ImageObject","inLanguage":"fr-CA","@id":"https:\/\/www.sygnaturediscovery.com\/fr\/case-study\/bk-channel-screening-%ce%b2-subunit-profiling\/#primaryimage","url":"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/Schematic-representation-of-the-BK-channel-structure-composed.gif","contentUrl":"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/Schematic-representation-of-the-BK-channel-structure-composed.gif"},{"@type":"BreadcrumbList","@id":"https:\/\/www.sygnaturediscovery.com\/fr\/case-study\/bk-channel-screening-%ce%b2-subunit-profiling\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/www.sygnaturediscovery.com\/fr\/page-daccueil\/"},{"@type":"ListItem","position":2,"name":"BK Channel Screening &amp; \u03b2-Subunit Profiling"}]},{"@type":"WebSite","@id":"https:\/\/www.sygnaturediscovery.com\/fr\/#website","url":"https:\/\/www.sygnaturediscovery.com\/fr\/","name":"Sygnature Discovery","description":"","publisher":{"@id":"https:\/\/www.sygnaturediscovery.com\/fr\/#organization"},"alternateName":"Sygnature","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.sygnaturediscovery.com\/fr\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"fr-CA"},{"@type":"Organization","@id":"https:\/\/www.sygnaturediscovery.com\/fr\/#organization","name":"Sygnature","url":"https:\/\/www.sygnaturediscovery.com\/fr\/","logo":{"@type":"ImageObject","inLanguage":"fr-CA","@id":"https:\/\/www.sygnaturediscovery.com\/fr\/#\/schema\/logo\/image\/","url":"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/11\/Logo.svg","contentUrl":"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/11\/Logo.svg","width":1,"height":1,"caption":"Sygnature"},"image":{"@id":"https:\/\/www.sygnaturediscovery.com\/fr\/#\/schema\/logo\/image\/"}}]}},"_links":{"self":[{"href":"https:\/\/www.sygnaturediscovery.com\/fr\/wp-json\/wp\/v2\/case-study\/6703","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.sygnaturediscovery.com\/fr\/wp-json\/wp\/v2\/case-study"}],"about":[{"href":"https:\/\/www.sygnaturediscovery.com\/fr\/wp-json\/wp\/v2\/types\/case-study"}],"version-history":[{"count":0,"href":"https:\/\/www.sygnaturediscovery.com\/fr\/wp-json\/wp\/v2\/case-study\/6703\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.sygnaturediscovery.com\/fr\/wp-json\/wp\/v2\/media?parent=6703"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.sygnaturediscovery.com\/fr\/wp-json\/wp\/v2\/category?post=6703"},{"taxonomy":"resource_tag","embeddable":true,"href":"https:\/\/www.sygnaturediscovery.com\/fr\/wp-json\/wp\/v2\/resource_tag?post=6703"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}