{"id":6492,"date":"2025-12-08T14:15:12","date_gmt":"2025-12-08T14:15:12","guid":{"rendered":"https:\/\/www.sygnaturediscovery.com\/?post_type=case-study&#038;p=6492"},"modified":"2025-12-15T00:37:04","modified_gmt":"2025-12-15T00:37:04","slug":"validating-a-high-throughput-ipsc-derived-drg-electrophysiology-platform","status":"publish","type":"case-study","link":"https:\/\/www.sygnaturediscovery.com\/fr\/case-study\/validating-a-high-throughput-ipsc-derived-drg-electrophysiology-platform\/","title":{"rendered":"Validating a High-Throughput iPSC-Derived DRG Electrophysiology Platform"},"content":{"rendered":"\n<h3 class=\"wp-block-heading has-dark-blue-500-color has-text-color has-link-color has-text-2-xl-font-size wp-elements-2154c44bc4ae466a82eceba10253547f\">Translational Relevance of iPSC-Derived Sensory Neurons<\/h3>\n\n\n\n<p>To address the critical need for scalable and human-relevant models in pain research, we validated an automated patch clamp platform using RealDRGx\u2122 iPSC-derived sensory neurons, which offer native-like electrophysiological behaviour and compatibility with high-throughput workflows. This platform is ideal for studying sensory neuron excitability and screening novel pharmacological agents targeting inflammatory and neuropathic pain. Dorsal root ganglion (DRG) neurons are key mediators of nociceptive signalling and are central to the development of analgesics. However, traditional primary neuron models are limited by variability and low throughput. Our iPSC-derived DRG system enables a reproducible, scalable alternative for preclinical screening.<\/p>\n\n\n\n<h3 class=\"wp-block-heading has-dark-blue-500-color has-text-color has-link-color has-text-2-xl-font-size wp-elements-a55a4d979770e27ab346952f1c7f7b64\">Six-Week Electrophysiological Profiling of RealDRGx\u2122 Neurons<\/h3>\n\n\n\n<p>RealDRGx\u2122 neurons consistently met quality control benchmarks, demonstrating high assay success rates and stable biophysical properties from week 3 onward\u2014supporting confident integration into screening cascades. Over six weeks of maturation, we monitored:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Seal resistance: Consistently &gt;200 M\u03a9<\/li>\n\n\n\n<li>Series resistance: Stable and &lt;20 M\u03a9<\/li>\n\n\n\n<li>Cell catch rate: &gt;90% by week 3<\/li>\n\n\n\n<li>Capacitance: Steady values, indicating membrane stability<\/li>\n<\/ul>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-66eaa2be wp-block-columns-is-layout-flex\" style=\"padding-bottom:var(--wp--preset--spacing--40)\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure data-wp-context='{\"imageId\":\"69f3b594f1421\"}' data-wp-interactive=\"core\/image\" data-wp-key=\"69f3b594f1421\" class=\"wp-block-image size-full has-custom-border wp-lightbox-container\" style=\"margin-top:var(--wp--preset--spacing--40);margin-bottom:var(--wp--preset--spacing--40)\"><img loading=\"lazy\" decoding=\"async\" width=\"593\" height=\"378\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/syncropatch-384-well-plate-assay-overview.webp\" alt=\"\" class=\"wp-image-6495\" style=\"border-radius:1.5em\" srcset=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/syncropatch-384-well-plate-assay-overview.webp 593w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/syncropatch-384-well-plate-assay-overview-300x191.webp 300w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/syncropatch-384-well-plate-assay-overview-565x360.webp 565w\" sizes=\"(max-width: 593px) 100vw, 593px\"><button class=\"lightbox-trigger\" type=\"button\" aria-haspopup=\"dialog\" aria-label=\"Agrandir\" data-wp-init=\"callbacks.initTriggerButton\" data-wp-on--click=\"actions.showLightbox\" data-wp-style--right=\"state.imageButtonRight\" data-wp-style--top=\"state.imageButtonTop\">\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewbox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\"><\/path>\n\t\t\t<\/svg>\n\t\t<\/button><\/figure>\n\n\n\n<h4 class=\"wp-block-heading has-blue-600-color has-text-color has-link-color has-text-lg-font-size wp-elements-e5f79dc365b23bacf42874d73a280c7e\">High-Throughput Electrophysiology<\/h4>\n\n\n\n<p>Our assay was developed on the SyncroPatch 384i platform, enabling efficient, automated whole-cell recordings. The screenshot highlights consistent seal formation and current traces across the plate, confirming the platform\u2019s suitability for scalable iPSC-derived DRG screening.<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure data-wp-context='{\"imageId\":\"69f3b594f3287\"}' data-wp-interactive=\"core\/image\" data-wp-key=\"69f3b594f3287\" class=\"wp-block-image size-full has-custom-border wp-lightbox-container\" style=\"margin-top:var(--wp--preset--spacing--40);margin-bottom:var(--wp--preset--spacing--40)\"><img loading=\"lazy\" decoding=\"async\" width=\"2560\" height=\"1711\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/drg-ipsc-series-resistance-weekly-scaled.webp\" alt=\"\" class=\"wp-image-6499\" style=\"border-radius:1.5em\" srcset=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/drg-ipsc-series-resistance-weekly-scaled.webp 2560w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/drg-ipsc-series-resistance-weekly-300x200.webp 300w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/drg-ipsc-series-resistance-weekly-1024x684.webp 1024w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/drg-ipsc-series-resistance-weekly-768x513.webp 768w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/drg-ipsc-series-resistance-weekly-1536x1026.webp 1536w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/drg-ipsc-series-resistance-weekly-1616x1080.webp 1616w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/drg-ipsc-series-resistance-weekly-539x360.webp 539w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/drg-ipsc-series-resistance-weekly-640x428.webp 640w\" sizes=\"(max-width: 2560px) 100vw, 2560px\"><button class=\"lightbox-trigger\" type=\"button\" aria-haspopup=\"dialog\" aria-label=\"Agrandir\" data-wp-init=\"callbacks.initTriggerButton\" data-wp-on--click=\"actions.showLightbox\" data-wp-style--right=\"state.imageButtonRight\" data-wp-style--top=\"state.imageButtonTop\">\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewbox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\"><\/path>\n\t\t\t<\/svg>\n\t\t<\/button><\/figure>\n\n\n\n<h4 class=\"wp-block-heading has-blue-600-color has-text-color has-link-color has-text-lg-font-size wp-elements-c2ca00fa6231a7376440b444d043a5e4\">Consistent Low Series Resistance<\/h4>\n\n\n\n<p>Series resistance remained stable and below the 20 M\u03a9 QC threshold throughout six weeks of iPSC-derived DRG neuron maturation. This ensures high-quality recordings and accurate voltage control- essential for precise ion channel characterization.<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure data-wp-context='{\"imageId\":\"69f3b594f3c1c\"}' data-wp-interactive=\"core\/image\" data-wp-key=\"69f3b594f3c1c\" class=\"wp-block-image size-full has-custom-border wp-lightbox-container\" style=\"margin-top:var(--wp--preset--spacing--40);margin-bottom:var(--wp--preset--spacing--40)\"><img loading=\"lazy\" decoding=\"async\" width=\"2560\" height=\"1710\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/drg-ipsc-seal-resistance-weekly-scaled.webp\" alt=\"\" class=\"wp-image-6500\" style=\"border-radius:1.5em\" srcset=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/drg-ipsc-seal-resistance-weekly-scaled.webp 2560w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/drg-ipsc-seal-resistance-weekly-300x200.webp 300w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/drg-ipsc-seal-resistance-weekly-1024x684.webp 1024w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/drg-ipsc-seal-resistance-weekly-768x513.webp 768w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/drg-ipsc-seal-resistance-weekly-1536x1026.webp 1536w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/drg-ipsc-seal-resistance-weekly-1617x1080.webp 1617w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/drg-ipsc-seal-resistance-weekly-539x360.webp 539w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/drg-ipsc-seal-resistance-weekly-640x428.webp 640w\" sizes=\"(max-width: 2560px) 100vw, 2560px\"><button class=\"lightbox-trigger\" type=\"button\" aria-haspopup=\"dialog\" aria-label=\"Agrandir\" data-wp-init=\"callbacks.initTriggerButton\" data-wp-on--click=\"actions.showLightbox\" data-wp-style--right=\"state.imageButtonRight\" data-wp-style--top=\"state.imageButtonTop\">\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewbox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\"><\/path>\n\t\t\t<\/svg>\n\t\t<\/button><\/figure>\n\n\n\n<h4 class=\"wp-block-heading has-blue-600-color has-text-color has-link-color has-text-lg-font-size wp-elements-b649b371793f30dea385a80b69781bb5\">Reliable Seal Formation<\/h4>\n\n\n\n<p>Seal resistance exceeded 200 M\u03a9 in over 85% of recorded cells each week, supporting robust electrophysiology access and high success rates. This consistency enables confident data generation throughout your discovery campaign.<\/p>\n<\/div>\n<\/div>\n\n\n\n<h3 class=\"wp-block-heading has-dark-blue-500-color has-text-color has-link-color has-text-2-xl-font-size wp-elements-1c25e366cca4c2c252db9434e90a25ff\">Pharmacological Sensitivity Across Ion Channel Classes<\/h3>\n\n\n\n<p>Studies confirmed that RealDRGx\u2122 sensory neurons express key voltage- and ligand-gated ion channels and respond reliably to standard tool compounds, supporting their use in pharmacological screening workflows. These results confirm functional expression and compound-responsiveness across targets relevant to pain signalling.<\/p>\n\n\n\n<div class=\"wp-block-group has-global-padding is-layout-constrained wp-block-group-is-layout-constrained\" allowchildrentofillwidth=\"\" overflow=\"\" flexwrap=\"\">\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-3d378c62 wp-block-columns-is-layout-flex\" style=\"margin-top:var(--wp--preset--spacing--40);margin-bottom:var(--wp--preset--spacing--40)\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure data-wp-context='{\"imageId\":\"69f3b5950069d\"}' data-wp-interactive=\"core\/image\" data-wp-key=\"69f3b5950069d\" class=\"wp-block-image size-full has-custom-border wp-lightbox-container\" style=\"margin-top:var(--wp--preset--spacing--40);margin-bottom:var(--wp--preset--spacing--40)\"><img loading=\"lazy\" decoding=\"async\" width=\"282\" height=\"190\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/drg-ipsc-kv-expression-over-time-e1744190625885.webp\" alt=\"\" class=\"wp-image-6508\" style=\"border-radius:1.5em\"><button class=\"lightbox-trigger\" type=\"button\" aria-haspopup=\"dialog\" aria-label=\"Agrandir\" data-wp-init=\"callbacks.initTriggerButton\" data-wp-on--click=\"actions.showLightbox\" data-wp-style--right=\"state.imageButtonRight\" data-wp-style--top=\"state.imageButtonTop\">\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewbox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\"><\/path>\n\t\t\t<\/svg>\n\t\t<\/button><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure data-wp-context='{\"imageId\":\"69f3b59500f1f\"}' data-wp-interactive=\"core\/image\" data-wp-key=\"69f3b59500f1f\" class=\"wp-block-image size-full has-custom-border wp-lightbox-container\" style=\"margin-top:var(--wp--preset--spacing--40);margin-bottom:var(--wp--preset--spacing--40)\"><img loading=\"lazy\" decoding=\"async\" width=\"296\" height=\"190\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/drg-ipsc-4ap-dose-response-kv-traces-e1744191268739.webp\" alt=\"\" class=\"wp-image-6510\" style=\"border-radius:1.5em\"><button class=\"lightbox-trigger\" type=\"button\" aria-haspopup=\"dialog\" aria-label=\"Agrandir\" data-wp-init=\"callbacks.initTriggerButton\" data-wp-on--click=\"actions.showLightbox\" data-wp-style--right=\"state.imageButtonRight\" data-wp-style--top=\"state.imageButtonTop\">\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewbox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\"><\/path>\n\t\t\t<\/svg>\n\t\t<\/button><\/figure>\n<\/div>\n<\/div>\n\n\n\n<p><strong><em>Figure 1: Functional Potassium Channel Expression and Pharmacological Modulation in iPSC-Derived DRG Neurons<\/em><\/strong><br>RealDRGx\u2122 sensory neurons demonstrate robust and sustained expression of functional voltage-gated potassium (Kv) channels, with over 90% of cell exhibiting Kv currents consistently from week 1 through week 6 (left). Cumulative current traces (right) show dose-dependent inhibition of Kv-mediated outward currents following application of 4-aminopyridine (4-AP), confirming pharmacological responsiveness and supporting their utility in ion channel screening workflows.<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-group has-global-padding is-layout-constrained wp-block-group-is-layout-constrained\" allowchildrentofillwidth=\"\" overflow=\"\" flexwrap=\"\">\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-60425b75 wp-block-columns-is-layout-flex\" style=\"margin-top:var(--wp--preset--spacing--40);margin-bottom:var(--wp--preset--spacing--40);padding-top:var(--wp--preset--spacing--40);padding-bottom:var(--wp--preset--spacing--40)\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure data-wp-context='{\"imageId\":\"69f3b5950175b\"}' data-wp-interactive=\"core\/image\" data-wp-key=\"69f3b5950175b\" class=\"wp-block-image size-full has-custom-border wp-lightbox-container\" style=\"margin-top:var(--wp--preset--spacing--40);margin-bottom:var(--wp--preset--spacing--40)\"><img loading=\"lazy\" decoding=\"async\" width=\"283\" height=\"190\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/syncropatch-384-well-plate-assay-overview-1.webp\" alt=\"\" class=\"wp-image-6514\" style=\"border-radius:1.5em\"><button class=\"lightbox-trigger\" type=\"button\" aria-haspopup=\"dialog\" aria-label=\"Agrandir\" data-wp-init=\"callbacks.initTriggerButton\" data-wp-on--click=\"actions.showLightbox\" data-wp-style--right=\"state.imageButtonRight\" data-wp-style--top=\"state.imageButtonTop\">\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewbox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\"><\/path>\n\t\t\t<\/svg>\n\t\t<\/button><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure data-wp-context='{\"imageId\":\"69f3b59501e93\"}' data-wp-interactive=\"core\/image\" data-wp-key=\"69f3b59501e93\" class=\"wp-block-image size-full has-custom-border wp-lightbox-container\" style=\"margin-top:var(--wp--preset--spacing--40);margin-bottom:var(--wp--preset--spacing--40)\"><img loading=\"lazy\" decoding=\"async\" width=\"218\" height=\"190\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/TTX-concentration-response-iPSC-DRG-e1744124296646.webp\" alt=\"\" class=\"wp-image-6515\" style=\"border-radius:1.5em\"><button class=\"lightbox-trigger\" type=\"button\" aria-haspopup=\"dialog\" aria-label=\"Agrandir\" data-wp-init=\"callbacks.initTriggerButton\" data-wp-on--click=\"actions.showLightbox\" data-wp-style--right=\"state.imageButtonRight\" data-wp-style--top=\"state.imageButtonTop\">\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewbox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\"><\/path>\n\t\t\t<\/svg>\n\t\t<\/button><\/figure>\n<\/div>\n<\/div>\n\n\n\n<p><strong><em>Figure 2: Sustained Sodium Channel Expression and TTX-Mediated Inhibition in iPSC-Drived DRG Neurons<\/em><\/strong><br>iPSC-derived DRG neurons exhibited stable expression of voltage-gated sodium (Nav) channels, with over 90% of cells showing functional sodium currents across 6 weeks (left). Representative current traces (right) illustrate dose-dependent inhibition of sodium currents by tetrodotoxin (TTX), confirming pharmacological responsiveness and functional Nav channel activity in the model system.<\/p>\n<\/div>\n\n\n\n<h3 class=\"wp-block-heading has-dark-blue-500-color has-text-color has-link-color has-text-2-xl-font-size wp-elements-384d4b928142bbe71acb38d71e57ed20\">Electrophysiology Excitability and Neuronal Firing<\/h3>\n\n\n\n<p>RealDRGx\u2122 neurons exhibited increasing excitability over time, including spontaneous and evoked firing that was sensitive to sodium channel blockade\u2014highlighting their functional maturity and suitability for screening. Key electrophysiological features included:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Resting membrane potential (RMP): Averaged between \u221240 to \u221250 mV<\/li>\n\n\n\n<li>Spontaneous firing: Frequency increased over the maturation period<\/li>\n\n\n\n<li>Evoked firing: Reliable responses to current injection from 0 to 200 pA<\/li>\n\n\n\n<li>TTX sensitivity: Clear inhibition of firing at nanomolar concentrations<\/li>\n\n\n\n<li>Progressive excitability: Enhanced firing behaviour from week 1 to 6<\/li>\n<\/ul>\n\n\n\n<div class=\"wp-block-group has-global-padding is-layout-constrained wp-block-group-is-layout-constrained\" allowchildrentofillwidth=\"\" overflow=\"\" flexwrap=\"\">\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-3d378c62 wp-block-columns-is-layout-flex\" style=\"margin-top:var(--wp--preset--spacing--40);margin-bottom:var(--wp--preset--spacing--40)\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure data-wp-context='{\"imageId\":\"69f3b59503180\"}' data-wp-interactive=\"core\/image\" data-wp-key=\"69f3b59503180\" class=\"wp-block-image size-full is-resized has-custom-border wp-lightbox-container\" style=\"margin-top:var(--wp--preset--spacing--40);margin-bottom:var(--wp--preset--spacing--40)\"><img loading=\"lazy\" decoding=\"async\" width=\"250\" height=\"190\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/drg-ipsc-evoked-firing-vi-protocol-e1744191465906.webp\" alt=\"\" class=\"wp-image-6523\" style=\"border-radius:1.5em;width:auto;height:90px\"><button class=\"lightbox-trigger\" type=\"button\" aria-haspopup=\"dialog\" aria-label=\"Agrandir\" data-wp-init=\"callbacks.initTriggerButton\" data-wp-on--click=\"actions.showLightbox\" data-wp-style--right=\"state.imageButtonRight\" data-wp-style--top=\"state.imageButtonTop\">\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewbox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\"><\/path>\n\t\t\t<\/svg>\n\t\t<\/button><\/figure>\n\n\n\n<h4 class=\"wp-block-heading has-blue-600-color has-text-color has-link-color has-text-lg-font-size wp-elements-ae006648b5033d3e4fba37bb26dd170e\">Progressive Increase in Evoked Firing Capacity<\/h4>\n\n\n\n<p>The percentage of DRG neurons capable of firing in response to depolarizing current injection increased over time, reaching over 90% by week 3, demonstrating functional excitability and assay readiness for evaluating neuronal firing modulation.<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure data-wp-context='{\"imageId\":\"69f3b59503aa2\"}' data-wp-interactive=\"core\/image\" data-wp-key=\"69f3b59503aa2\" class=\"wp-block-image size-full is-resized has-custom-border wp-lightbox-container\" style=\"margin-top:var(--wp--preset--spacing--40);margin-bottom:var(--wp--preset--spacing--40)\"><img loading=\"lazy\" decoding=\"async\" width=\"347\" height=\"217\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/iPSC-DRG-action-potential-TTX-inhibition.webp\" alt=\"\" class=\"wp-image-6525\" style=\"border-radius:1.5em;width:auto;height:90px\" srcset=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/iPSC-DRG-action-potential-TTX-inhibition.webp 347w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/iPSC-DRG-action-potential-TTX-inhibition-300x188.webp 300w\" sizes=\"(max-width: 347px) 100vw, 347px\"><button class=\"lightbox-trigger\" type=\"button\" aria-haspopup=\"dialog\" aria-label=\"Agrandir\" data-wp-init=\"callbacks.initTriggerButton\" data-wp-on--click=\"actions.showLightbox\" data-wp-style--right=\"state.imageButtonRight\" data-wp-style--top=\"state.imageButtonTop\">\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewbox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\"><\/path>\n\t\t\t<\/svg>\n\t\t<\/button><\/figure>\n\n\n\n<h4 class=\"wp-block-heading has-blue-600-color has-text-color has-link-color has-text-lg-font-size wp-elements-957541ed541ae5e9b426e61506de78cd\">TTX-Sensitive Action Potential Firing<\/h4>\n\n\n\n<p>Representative voltage traces show evoked action potentials under control conditions and following application of 500 nM TTX. The clear inhibition confirms functional sodium channel activity and the platform\u2019s suitability for screening excitability-modulating compounds.<\/p>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<h3 class=\"wp-block-heading has-dark-blue-500-color has-text-color has-link-color has-text-2-xl-font-size wp-elements-9c0603037e134c6bf5096338be1e0134\">A Validated, Scalable Platform for Ion Channel-Targeting Pain Drug Discovery<\/h3>\n\n\n\n<p>Our iPSC-derived DRG neuron electrophysiology platform offers:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>A\u00a0<strong>scalable, human-relevant alternative<\/strong>\u00a0to primary DRG models<\/li>\n\n\n\n<li><strong>Automated, high-throughput compatibility<\/strong>\u00a0using the SyncroPatch 384i<\/li>\n\n\n\n<li><strong>Pharmacological validation<\/strong>\u00a0across key ion channels and receptors<\/li>\n\n\n\n<li><strong>Functionally mature sensory neurons<\/strong>, ideal for pain-focused drug discovery<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"","protected":false},"featured_media":0,"template":"","category":[707,773,775,746,780,778],"resource_tag":[],"class_list":["post-6492","case-study","type-case-study","status-publish","hentry","category-electrophysiology","category-gabaa-receptors","category-glycine-receptors","category-ion-channels","category-potassium-channels","category-sodium-channels"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - 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