{"id":6166,"date":"2025-12-05T13:51:25","date_gmt":"2025-12-05T13:51:25","guid":{"rendered":"https:\/\/www.sygnaturediscovery.com\/?post_type=case-study&p=6166"},"modified":"2026-02-05T16:44:54","modified_gmt":"2026-02-05T16:44:54","slug":"sygnature-discoverys-mouse-formalin-model","status":"publish","type":"case-study","link":"https:\/\/www.sygnaturediscovery.com\/fr\/case-study\/sygnature-discoverys-mouse-formalin-model\/","title":{"rendered":"Sygnature Discovery\u2019s Mouse-Formalin Model"},"content":{"rendered":"\n

The mouse-formalin pain model stands as a chemically-evoked acute pain model within our research arsenal at Sygnature Discovery.\u00a0<\/h2>\n\n\n\n

Well-known for its capability for rapid screening, it\u2019s particularly well-suited for most analgesic targets. The model delves into both central and peripheral mechanisms of pain, with its Phase 2 often regarded as a \u2018surrogate\u2019 for neuropathic pain models, thereby expanding its utility.<\/p>\n\n\n\n

The induction of this model involves the careful injection of a diluted formalin solution into the mouse\u2019s paw. Post-injection, observable behaviors such as biting, licking, and guarding provide crucial indicators of pain reactions. The model\u2019s response is classified into two distinct phases, 0-5 minutes representing Phase 1, and the duration from 15-60 minutes designated as Phase 2 while 5-15 minutes represents a quiescent interphase.<\/p>\n\n\n\n

Sygnature Discovery\u2019s standard study design for this model generally comprises 4-6 groups, with each group containing 10 subjects to maintain data integrity and robustness. A range of positive controls, including Duloxetine, Gabapentin, Mexiletine, and Diclofenac, have been tested to ascertain the model\u2019s reliability and scope. To ensure consistent results and uphold the highest standards of scientific investigation, the model is meticulously validated for the C57BL6 mouse species.<\/p>\n\n\n\n

In the comprehensive evaluation of the mouse-formalin pain model, a salient observation is the pronounced pain response exhibited by male mice compared with the females, a factor that underpins our choice to utilize males for the model. Their heightened sensitivity offers a more robust platform to discern and quantify pain reactions, making the analysis more nuanced and informative.<\/p>\n\n\n\n

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