{"id":5125,"date":"2025-12-02T16:34:22","date_gmt":"2025-12-02T16:34:22","guid":{"rendered":"https:\/\/www.sygnaturediscovery.com\/?post_type=case-study&#038;p=5125"},"modified":"2026-02-05T17:01:08","modified_gmt":"2026-02-05T17:01:08","slug":"when-hplc-fails","status":"publish","type":"case-study","link":"https:\/\/www.sygnaturediscovery.com\/fr\/case-study\/when-hplc-fails\/","title":{"rendered":"When HPLC Fails: SFC Saves the Day"},"content":{"rendered":"\n<h3 class=\"wp-block-heading has-dark-blue-500-color has-text-color has-link-color has-text-2-xl-font-size wp-elements-caf54642d97c8a771a10dc4edf505c2c\">Case A \u2013 Solubility barrier<\/h3>\n\n\n\n<p>A compound\u2019s solubility prevented HPLC analysis. We used a\u00a0<strong>DCM\/MeOH mix<\/strong>\u00a0and transitioned to\u00a0<strong>analytical SFC (UPC\u00b2)<\/strong>.<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Excellent peak shape<\/li>\n\n\n\n<li>Retention time adapted for preparative scale<\/li>\n\n\n\n<li>Seamless handoff to prep-SFC<\/li>\n<\/ul>\n\n\n\n<figure data-wp-context='{\"imageId\":\"69f30a242fbd8\"}' data-wp-interactive=\"core\/image\" data-wp-key=\"69f30a242fbd8\" class=\"wp-block-image alignleft size-full has-custom-border is-style-default wp-lightbox-container\"><img loading=\"lazy\" decoding=\"async\" width=\"916\" height=\"368\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-4-1.webp\" alt=\"\" class=\"wp-image-5127\" style=\"border-radius:1.5em\" srcset=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-4-1.webp 916w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-4-1-300x121.webp 300w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-4-1-768x309.webp 768w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-4-1-640x257.webp 640w\" sizes=\"(max-width: 916px) 100vw, 916px\"><button class=\"lightbox-trigger\" type=\"button\" aria-haspopup=\"dialog\" aria-label=\"Agrandir\" data-wp-init=\"callbacks.initTriggerButton\" data-wp-on--click=\"actions.showLightbox\" data-wp-style--right=\"state.imageButtonRight\" data-wp-style--top=\"state.imageButtonTop\">\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewbox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\"><\/path>\n\t\t\t<\/svg>\n\t\t<\/button><\/figure>\n\n\n\n<div style=\"height:84px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading has-dark-blue-500-color has-text-color has-link-color has-text-2-xl-font-size wp-elements-2b9e4f1ffa94e04b19e13eda10b4b789\">Case B \u2013 Apolar compound tailing in RP-HPLC<\/h3>\n\n\n\n<p>The compound produced tailing peaks under reverse-phase HPLC. Switched to\u00a0<strong>achiral SFC<\/strong>, improving both separation and speed.<\/p>\n\n\n\n<figure data-wp-context='{\"imageId\":\"69f30a243078b\"}' data-wp-interactive=\"core\/image\" data-wp-key=\"69f30a243078b\" class=\"wp-block-image alignleft size-full has-custom-border is-style-default wp-lightbox-container\"><img loading=\"lazy\" decoding=\"async\" width=\"890\" height=\"378\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-4-2.webp\" alt=\"\" class=\"wp-image-5128\" style=\"border-radius:1.5em\" srcset=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-4-2.webp 890w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-4-2-300x127.webp 300w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-4-2-768x326.webp 768w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-4-2-640x272.webp 640w\" sizes=\"(max-width: 890px) 100vw, 890px\"><button class=\"lightbox-trigger\" type=\"button\" aria-haspopup=\"dialog\" aria-label=\"Agrandir\" data-wp-init=\"callbacks.initTriggerButton\" data-wp-on--click=\"actions.showLightbox\" data-wp-style--right=\"state.imageButtonRight\" data-wp-style--top=\"state.imageButtonTop\">\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewbox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\"><\/path>\n\t\t\t<\/svg>\n\t\t<\/button><\/figure>\n\n\n\n<div style=\"height:42px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading has-dark-blue-500-color has-text-color has-link-color has-text-2-xl-font-size wp-elements-674274f2b10218c851f7d0cdf8be4477\">From Discovery to Preclinical\u2014and Beyond<\/h3>\n\n\n\n<p>For our clients, the benefits of Sygnature\u2019s separation sciences platform are clear:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Speed<\/strong>: High-purity materials generated faster, even at scale<\/li>\n\n\n\n<li><strong>Sustainability<\/strong>: Less solvent use, greener workflows<\/li>\n\n\n\n<li><strong>Cost efficiency<\/strong>: No unnecessary GMP-grade overengineering in early phases<\/li>\n\n\n\n<li><strong>Risk reduction<\/strong>: In-house method development and continuity through scale-up<\/li>\n\n\n\n<li><strong>Clinical foresight<\/strong>: Prep methods transferable to CDMOs and GLP\/GMP partners<\/li>\n<\/ul>\n\n\n\n<p>We\u2019re not just solving analytical puzzles, we\u2019re preparing you for what\u2019s next, with\u00a0<strong>data and material you can act on<\/strong>.<\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"featured_media":0,"template":"","category":[682,717],"resource_tag":[],"class_list":["post-5125","case-study","type-case-study","status-publish","hentry","category-chemistry","category-separation-sciences"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - 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