{"id":5039,"date":"2025-12-02T16:00:20","date_gmt":"2025-12-02T16:00:20","guid":{"rendered":"https:\/\/www.sygnaturediscovery.com\/?post_type=case-study&#038;p=5039"},"modified":"2026-02-05T17:02:45","modified_gmt":"2026-02-05T17:02:45","slug":"hplc-prep-one-run-five-isomers","status":"publish","type":"case-study","link":"https:\/\/www.sygnaturediscovery.com\/fr\/case-study\/hplc-prep-one-run-five-isomers\/","title":{"rendered":"HPLC Prep: One Run, Five Isomers, All Pure"},"content":{"rendered":"\n<h3 class=\"wp-block-heading has-dark-blue-500-color has-text-color has-link-color has-text-3-xl-font-size wp-elements-ca76e401fb4c99bba857e622185bb2d3\">Challenge : A client needed to isolate five closely related isomers with \u226590% purity for NMR and LCMS analysis.<\/h3>\n\n\n\n<figure class=\"wp-block-image alignfull size-full has-custom-border\"><img loading=\"lazy\" decoding=\"async\" width=\"1070\" height=\"296\" src=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-1.webp\" alt=\"\" class=\"wp-image-5055\" style=\"border-radius:1em\" srcset=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-1.webp 1070w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-1-300x83.webp 300w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-1-1024x283.webp 1024w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-1-768x212.webp 768w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-1-640x177.webp 640w\" sizes=\"(max-width: 1070px) 100vw, 1070px\"><\/figure>\n\n\n\n<div style=\"height:42px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div style=\"height:42px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-28f84493 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image alignleft size-full has-custom-border is-style-default\" style=\"margin-top:var(--wp--preset--spacing--20);margin-bottom:var(--wp--preset--spacing--20)\"><img loading=\"lazy\" decoding=\"async\" width=\"224\" height=\"509\" src=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-7.webp\" alt=\"\" class=\"wp-image-5076\" style=\"border-radius:1em\" srcset=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-7.webp 224w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-7-132x300.webp 132w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-7-158x360.webp 158w\" sizes=\"(max-width: 224px) 100vw, 224px\"><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<h3 class=\"wp-block-heading has-dark-blue-500-color has-text-color has-link-color has-text-2-xl-font-size wp-elements-a9224590df9d5968a33c7f36e4efde41\">Approach<\/h3>\n\n\n\n<p>Using an\u00a0<strong>X-Select column<\/strong>\u00a0with a 55\u201375% ACN\/H\u2082O (+FA) gradient, we achieved full separation in\u00a0<strong>a single purification run<\/strong>.<\/p>\n\n\n\n<h3 class=\"wp-block-heading has-dark-blue-500-color has-text-color has-link-color has-text-2-xl-font-size wp-elements-55aa61a409a30bdbf5b281a8192a18be\">Outcome<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>All five isomers were obtained with &gt;95% purity<\/li>\n\n\n\n<li>Time and solvent use were minimized<\/li>\n\n\n\n<li>A complex challenge resolved in one efficient step<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<figure class=\"wp-block-image alignfull size-full has-custom-border\" style=\"margin-top:var(--wp--preset--spacing--20);margin-bottom:var(--wp--preset--spacing--20)\"><img loading=\"lazy\" decoding=\"async\" width=\"1019\" height=\"286\" src=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-2.webp\" alt=\"\" class=\"wp-image-5061\" style=\"border-radius:1em\" srcset=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-2.webp 1019w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-2-300x84.webp 300w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-2-768x216.webp 768w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-2-640x180.webp 640w\" sizes=\"(max-width: 1019px) 100vw, 1019px\"><\/figure>\n\n\n\n<figure class=\"wp-block-image alignfull size-full has-custom-border\" style=\"margin-top:var(--wp--preset--spacing--20);margin-bottom:var(--wp--preset--spacing--20)\"><img loading=\"lazy\" decoding=\"async\" width=\"1014\" height=\"278\" src=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-3.webp\" alt=\"\" class=\"wp-image-5062\" style=\"border-radius:1em\" srcset=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-3.webp 1014w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-3-300x82.webp 300w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-3-768x211.webp 768w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-3-640x175.webp 640w\" sizes=\"(max-width: 1014px) 100vw, 1014px\"><\/figure>\n\n\n\n<figure class=\"wp-block-image alignfull size-full has-custom-border\" style=\"margin-top:var(--wp--preset--spacing--20);margin-bottom:var(--wp--preset--spacing--20)\"><img loading=\"lazy\" decoding=\"async\" width=\"1016\" height=\"277\" src=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-4.webp\" alt=\"\" class=\"wp-image-5064\" style=\"border-radius:1em\" srcset=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-4.webp 1016w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-4-300x82.webp 300w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-4-768x209.webp 768w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-4-640x174.webp 640w\" sizes=\"(max-width: 1016px) 100vw, 1016px\"><\/figure>\n\n\n\n<figure class=\"wp-block-image alignfull size-full has-custom-border\" style=\"margin-top:var(--wp--preset--spacing--20);margin-bottom:var(--wp--preset--spacing--20)\"><img loading=\"lazy\" decoding=\"async\" width=\"1014\" height=\"300\" src=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-5.webp\" alt=\"\" class=\"wp-image-5065\" style=\"border-radius:1em\" srcset=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-5.webp 1014w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-5-300x89.webp 300w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-5-768x227.webp 768w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-5-640x189.webp 640w\" sizes=\"(max-width: 1014px) 100vw, 1014px\"><\/figure>\n\n\n\n<figure class=\"wp-block-image alignfull size-full has-custom-border\" style=\"margin-top:var(--wp--preset--spacing--20);margin-bottom:var(--wp--preset--spacing--20)\"><img loading=\"lazy\" decoding=\"async\" width=\"1012\" height=\"280\" src=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-6.webp\" alt=\"\" class=\"wp-image-5066\" style=\"border-radius:1em\" srcset=\"https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-6.webp 1012w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-6-300x83.webp 300w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-6-768x212.webp 768w, https:\/\/www.sygnaturediscovery.com\/wp-content\/uploads\/2025\/12\/SepSci_Example-1-6-640x177.webp 640w\" sizes=\"(max-width: 1012px) 100vw, 1012px\"><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>WO 2025057106 A1 &#038; J. Med. Chem.<br \/>\n2024, 67, 7, 10251-10284<\/p>\n","protected":false},"featured_media":0,"template":"","category":[682,717],"resource_tag":[],"class_list":["post-5039","case-study","type-case-study","status-publish","hentry","category-chemistry","category-separation-sciences"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>HPLC Prep: One Run, Five Isomers, All Pure - Sygnature<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.sygnaturediscovery.com\/fr\/case-study\/hplc-prep-one-run-five-isomers\/\" \/>\n<meta property=\"og:locale\" content=\"fr_CA\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"HPLC Prep: One Run, Five Isomers, All Pure - Sygnature\" \/>\n<meta property=\"og:description\" content=\"WO 2025057106 A1 &amp; J. 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