{"id":16951,"date":"2024-01-22T14:49:00","date_gmt":"2024-01-22T14:49:00","guid":{"rendered":"https:\/\/www.sygnaturediscovery.com\/blog\/unveiling-cellular-secrets-how-does-automated-microscopy-expedite-imaging-in-cell-biology\/"},"modified":"2024-01-22T14:49:00","modified_gmt":"2024-01-22T14:49:00","slug":"unveiling-cellular-secrets-how-does-automated-microscopy-expedite-imaging-in-cell-biology","status":"publish","type":"blog","link":"https:\/\/www.sygnaturediscovery.com\/fr\/blog\/unveiling-cellular-secrets-how-does-automated-microscopy-expedite-imaging-in-cell-biology\/","title":{"rendered":"How Does Automated Microscopy Expedite Imaging in Cell Biology?"},"content":{"rendered":"\n

In the vast realm of cell biology, innovative methods and pioneering technologies continuously redefine the boundaries of our knowledge. The marriage of automated microscopy and computational analysis has given rise to high-content imaging, a powerful tool that allows for intricate visual insights into cellular processes. Our recent study examined the nuances of high-content imaging using the highly proficient EVOS\u2122 M7000 instrument.<\/p>\n\n\n\n

Quantification of Pharmacochaperone Expression at the Cellular Level<\/strong><\/h3>\n\n\n\n

Pharmacochaperones play a pivotal role in rescuing the activity of misfolded target proteins. A significant aspect of our research was directed at the quantification of a receptor trafficked to the cellular membrane using a pharmacochaperone in mutant cell lines. The EVOS\u2122 M7000 was instrumental in providing quick high-resolution acquisition across an entire 384 well plate, allowing high throughput quantification of pharmacochaperone activity.<\/p>\n\n\n\n

Toxicity Evaluation Simplified<\/h3>\n\n\n\n

The realm of compound toxicity evaluation underwent a paradigm shift with our use of Automated Microscopy. Gone are the days when additional cell viability assays were a necessity. Our results demonstrated that merely scanning assay plates suffices to detect compound toxicity, thereby streamlining the process and ensuring faster results.<\/p>\n\n\n\n

Enzymatic Activity Assay with a Twist<\/h3>\n\n\n\n

Enzymes, the biocatalysts of the cellular world, were put under the microscope \u2014 quite literally. We employed a fluorescent substrate to detect enzyme activity, followed by a meticulous examination using the Cellesteto ascertain if the enzyme activity showcased a reduced vesicular pattern of expression following inhibition by a small molecule. Our comparison between the Wild Type (WT) and Knockout (KO) cell line using this fluorescent substrate, once again with Automated Microscopy, confirmed specificity of the substrate to the enzyme activity.<\/p>\n\n\n\n

Detecting Proteins Expressional Nuances<\/h3>\n\n\n\n

Using an immunofluorescence assay, the expression of protein A and its vesicular localization was quantified across an entire plate. The EVOS\u2122 M7000, with its automated imaging capabilities, allows to image, quantify protein expression and localization over multiple conditions simultaneously<\/p>\n\n\n\n

Key Takeaways and A Peek into the Future<\/h3>\n\n\n\n
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  1. The proficiency of the EVOS\u2122 M7000 is undeniable, with its capability to acquire images from an entire 96 or 384 well-plate in a mere 5 minutes.<\/li>\n\n\n\n
  2. The EVOS\u2122 M7000 facilitates quick and high throughout analysis of compound toxicity without additional costs, courtesy of the scanning capabilities of assay plates.<\/li>\n\n\n\n
  3. Swiftly visualize protein expression across various conditions with our automated microscopy technology. The Celleste 6\u2122 analysis software has proven to be incredibly versatile, playing a crucial role in a wide range of applications, including cell viability, apoptosis, and cell cycle analysis.<\/li>\n<\/ol>\n\n\n\n

    The Call to Arms<\/h3>\n\n\n\n