{"id":16907,"date":"2025-08-22T16:42:58","date_gmt":"2025-08-22T16:42:58","guid":{"rendered":"https:\/\/www.sygnaturediscovery.com\/blog\/advanced-separation-sciences-drive-discovery-at-sygnature\/"},"modified":"2025-08-22T16:42:58","modified_gmt":"2025-08-22T16:42:58","slug":"advanced-separation-sciences-drive-discovery-at-sygnature","status":"publish","type":"blog","link":"https:\/\/www.sygnaturediscovery.com\/fr\/blog\/advanced-separation-sciences-drive-discovery-at-sygnature\/","title":{"rendered":"From Analytical to Kilogram:How Advanced Separation Sciences Drive Discovery at Sygnature\u00a0"},"content":{"rendered":"\n

In modern drug discovery, speed and scale are everything. But achieving both, without compromising quality, requires more than great chemistry. It demands exceptional analytical and separation sciences, seamlessly integrated with your program from the very start.<\/h2>\n\n\n\n

At Sygnature Discovery, this is the standard we set.<\/p>\n\n\n\n

Our separation sciences platform, featuring\u00a0state-of-the-art SFC and HPLC technologies<\/strong>, is built to support your project through every stage of its journey. Whether you need a single isomer in high purity or 100 grams of a preclinical candidate, we don\u2019t just deliver, we design the most efficient, cost-effective path to get you there.<\/p>\n\n\n\n

Because for us, purification isn\u2019t just a technical step.\u00a0It\u2019s a\u00a0strategic advantage<\/strong>.<\/p>\n\n\n\n

The Right Tool for the Right Molecule: HPLC vs. SFC<\/h3>\n\n\n\n

Our team approaches purification with the same mindset we bring to medicinal chemistry: one size never fits all. That\u2019s why we leverage both\u00a0high-performance liquid chromatography (HPLC)<\/strong>\u00a0and\u00a0supercritical fluid chromatography (SFC)<\/strong>, switching between them or combining both based on your molecule\u2019s physical properties and the phase of your project.<\/p>\n\n\n\n

Here\u2019s how we compare them:<\/p>\n\n\n

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\n<\/div>\n\t\t\t\t\t\n\t\t\t
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\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n
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\n Feature\n <\/div>\n <\/th>\n
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\n HPLC\n <\/div>\n <\/th>\n
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\n SFC\n <\/div>\n <\/th>\n <\/tr>\n <\/thead>\n \n
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Solvent System<\/strong><\/p>\n\n <\/div>\n <\/div>\n <\/td>\n \n \n \n \n \n

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Organic + aqueous<\/p>\n\n <\/div>\n <\/div>\n <\/td>\n \n \n \n \n \n

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CO\u2082-based + organic modifier<\/p>\n\n <\/div>\n <\/div>\n <\/td>\n <\/tr>\n

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Chiral separations<\/strong><\/p>\n\n <\/div>\n <\/div>\n <\/td>\n \n \n \n \n \n

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Yes (longer runtimes)<\/p>\n\n <\/div>\n <\/div>\n <\/td>\n \n \n \n \n \n

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Yes (faster, more efficient)<\/p>\n\n <\/div>\n <\/div>\n <\/td>\n <\/tr>\n

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Speed<\/strong><\/p>\n\n <\/div>\n <\/div>\n <\/td>\n \n \n \n \n \n

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Slower<\/p>\n\n <\/div>\n <\/div>\n <\/td>\n \n \n \n \n \n

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Rapid separation and shorter RT<\/p>\n\n <\/div>\n <\/div>\n <\/td>\n <\/tr>\n

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Solvent consumption<\/strong><\/p>\n\n <\/div>\n <\/div>\n <\/td>\n \n \n \n \n \n

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High<\/p>\n\n <\/div>\n <\/div>\n <\/td>\n \n \n \n \n \n

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Low (green and cost-efficient)<\/p>\n\n <\/div>\n <\/div>\n <\/td>\n <\/tr>\n

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Best for\u2026<\/strong><\/p>\n\n <\/div>\n <\/div>\n <\/td>\n \n \n \n \n \n

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Polar & aqueous molecules<\/p>\n\n <\/div>\n <\/div>\n <\/td>\n \n \n \n \n \n

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Lipophilic, chiral, or apolar molecules<\/p>\n\n <\/div>\n <\/div>\n <\/td>\n <\/tr>\n

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Scale-up readiness<\/strong><\/p>\n\n <\/div>\n <\/div>\n <\/td>\n \n \n \n \n \n

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Standard<\/p>\n\n <\/div>\n <\/div>\n <\/td>\n \n \n \n \n \n

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Excellent via 30\u201350 mm prep columns<\/p>\n\n <\/div>\n <\/div>\n <\/td>\n <\/tr>\n <\/tbody>\n\n <\/table>\n <\/div>\n<\/div>\n\n<\/div>\n\t\t\n\t <\/section>\n\n\n\n

At Sygnature, this strategic flexibility allows us to consistently\u00a0save time, reduce solvent use, and optimize resolution,\u00a0<\/strong>from the first microgram to the last kilo.<\/p>\n\n\n

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Case-Backed Science: What This Means for Your Project<\/h2>\n

Let\u2019s walk through what this looks like in practice, with real examples from our labs.<\/p>\n\n\n\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t

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