DMPK and Physical Sciences

The DMPK and Physical Sciences Department is dedicated to the understanding of the absorption, distribution, metabolism and excretion of drug candidates. Our industry experienced scientists work in close partnership with the client and other departments within Sygnature.

We deliver valuable and reproducible DMPK data using industry standard assay technologies and techniques that support an efficient design, make, test, analyse cycle during an integrated project.

Our scientists not only undertake the in vitro assays, but also have the expertise to interpret the data generated. They also have the know-how and experience to provide a comprehensive consultancy service to interpret in vitro DMPK and in vivo PK data generated elsewhere. In addition, throughout the course of a project our clients have direct access to our scientists to facilitate complete transparency and detailed discussions.


Examples

The following are examples of DMPK and Physical Sciences assays which typically form part of an integrated drug discovery project undertaken at Sygnature:

    Physico-chemical profiling

    • Solubility (kinetic), LogD (micro shake-flask), pKa, PAMPA, Chemical stability

    Absorption

    • Caco-2, MDCK
    • Transporter inhibition

    Distribution

    • Plasma protein binding (rapid equilibrium dialysis) (RED device)
    • Blood-plasma partitioning
    • CNS/Brain exposure

    Metabolism

    • Microsomal stability (Clint & t1/2)
    • Hepatocyte stability (Clint & t1/2)
    • Plasma stability (t1/2)
    • Metabolite profiling and identification (LC-MS-TOF based)

    Bioanalysis and Pharmacokinetics

    • Support PK studies (in-life phase outsourced)
    • PK/PD relationships and dose predictions

    In-house instrumentation

    • Waters Xevo G2-XS QTOF (UPLC-MS-TOF)
    • Waters Xevo TQS-micro (UPLC-MS-MS)
    • Waters Acquity QDa (UPLC-MS)
    • MD SpectraMax 384 (analyte detection)
    • Agilent HP1100/1200 (HPLC +/- MS Systems)
    • Waters Semi-Prep (HPLC)
    • Beckman Biomek NXP
    • Genevac EZ2-Plus evaporator

DMPK Posters available upon request


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